STAT3 Antibody - N-terminal region (ARP38253_P050)

• Product Number: ARP38253_P050
• Name: STAT3 Antibody - N-terminal region (ARP38253_P050)
• Protein Size (# AA): 769 amino acids
• Molecular Weight: 88 kDa
• NCBI Gene Id: 6774
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Signal transducer and activator of transcription 3 (acute-phase response factor)
• Alias Symbols: APRF, HIES, ADMIO, ADMIO1
• Peptide Sequence: Synthetic peptide located within the following region: AQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKES
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ohbayashi,N., (2008) Biochem. Biophys. Res. Commun. 371 (4), 823-828
• Description of Target: STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
• Protein Interactions: MTOR; GADD45A; BMX; BLK; UBC; SYK; LMO2; SRI; HIC1; GNB2; USP22; RELA; COPS5; BRD4; ADRB2; PSMG3; TBCD; TBC1D15; PUS7; LUC7L2; ARFIP1; IVNS1ABP; MSH2; BMH1; SIN3A; EGFR; PIAS3; HDAC3; SRC; LYN; JAK3; JAK2; HES5; TRIM28; HES1; HCK; FYN; FGR; HNRNPM; SULT2A
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR	Y
  YCHAROS

• Additional Information: IHC Information: Kidney
  IHC Information: Uterus
  IHC Information: Prostate
• Other Applications Image 1 Data: Immunofluorescence --
  Sample Type: Macrophages
  Dilution: 1:1000
• Blocking Peptide: For anti-STAT3 (ARP38253_P050) antibody is Catalog # AAP38253 (Previous Catalog # AAPP23107)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human STAT3
• Uniprot ID: B5BTZ6
• Protein Name: Signal transducer and activator of transcription 3 isoform 2 EMBL BAG70046.1
• Publications:

  Bone marrow mesenchymal stromal cells with CD47 high expression via the signal transducer and activators of transcription signaling pathway preventing myocardial fibrosis. Int J Clin Exp Pathol. 8, 10555-64 (2015). 26617765

  Deng, W. et al. Bone marrow mesenchymal stromal cells with support of bispecific antibody and ultrasound-mediated microbubbles prevent myocardial fibrosis via the signal transducer and activators of transcription signaling pathway. Cytotherapy 13, 431-40 (2011). 21174489

  Vega, V. L. et al. Activation of the stress response in macrophages alters the M1/M2 balance by enhancing bacterial killing and IL-10 expression. J. Mol. Med. (Berl). doi:10.1007/s00109-014-1201-y (2014). 25163764

• Protein Accession #: NP_003141
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003150
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: STAT3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB, IF
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
• Image 1: human prostate
  
  Immunohistochemistry with human prostate tissue tissue
• Image 2: Human Uterus
  
  Immunohistochemistry with Human Uterus lysate tissue
• Image 3: Human kidney
  
  Immunohistochemistry with Human kidney lysate tissue
• Image 4: A549, HepG2
  
  Host: Rabbit
  Target: STAT3
  Positive control (+): A549 (N03)
  Negative control (-): HepG2 (HG)
  Antibody concentration: 1ug/ml
• Image 5: Mouse
  
  Immunofluorescence --Sample Type: Macrophages
  Dilution: 1:1000
• Image 6: Human Liver
  
  WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Human Liver
• Image 7:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.