E2F3 Antibody - C-terminal region (ARP38089_P050)

• Product Number: ARP38089_P050
• Name: E2F3 Antibody - C-terminal region (ARP38089_P050)
• Protein Size (# AA): 465 amino acids
• Molecular Weight: 49 kDa
• NCBI Gene Id: 1871
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: E2F transcription factor 3
• Alias Symbols: E2F-3
• Peptide Sequence: Synthetic peptide located within the following region: LLQQTEDQIPSNLEGPFVNLLPPLLQEDYLLSLGEEEGISDLFDAYDLEK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Koehrer,K., et al., (2004) The German cDNA Consortium
• Description of Target: E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
• Protein Interactions: RB1; TRIM28; TFDP1; HDAC1; SRA1; RBL1; FZR1; ELAVL1; PCNA; MCM4; HELLS; CCNB1; BCL6; ATAD2; HCFC1; USF1; KMT2D; RYBP; TFDP2; YY1; TFE3; SP1; CCNA2; CDK3; RNF144A; FHL2; WNK1; MGA; TEAD3; SPIB; PKIB; MYBL2; CREBBP; EAPP; JMY; PPP1R13B; TP53BP2; TP53INP1; B
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Specificity: The immunizing peptide used to raise this antibody is 100% homologous to isoform 2 (334aa, 37kDa) of human E2F3.
• Blocking Peptide: For anti-E2F3 (ARP38089_P050) antibody is Catalog # AAP38089 (Previous Catalog # AAPP20263)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human E2F3
• Uniprot ID: Q68DT0
• Protein Name: Putative uncharacterized protein DKFZp686C18211 EMBL CAH18140.1
• Publications:

  Giangreco, A. A. et al. Tumor suppressor microRNAs, miR-100 and -125b, are regulated by 1,25-dihydroxyvitamin D in primary prostate cells and in patient tissue. Cancer Prev. Res. (Phila). 6, 483-94 (2013). 23503652

  Melcher, R. et al. LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability. Carcinogenesis 32, 636-42 (2011). 21297112

  Naga Prasad, S. V et al. Unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks. J. Biol. Chem. 284, 27487-99 (2009). 19641226

• Protein Accession #: CAH18140
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001243076
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: E2F3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100%
• Image 1: Mouse Small Intestine
  
  Host: Mouse
  Target Name: E2F3
  Sample Tissue: Mouse Small Intestine
  Antibody Dilution: 1ug/ml
• Image 2: Human Ovary Tumor
  
  Host: Rabbit
  Target Name: E2F3
  Sample Tissue: Human Ovary Tumor
  Antibody Dilution: 1.0ug/ml
• Image 3: Human OVCAR-3 Whole Cell
  
  Host: Rabbit
  Target Name: E2F3
  Sample Tissue: Human OVCAR-3 Whole Cell
  Antibody Dilution: 1ug/ml