ID4 Antibody - N-terminal region (ARP38058_T100)

Data Sheet
 
Product Number ARP38058_T100
Product Page www.avivasysbio.com/id4-antibody-n-terminal-region-arp38058-t100.html
Name ID4 Antibody - N-terminal region (ARP38058_T100)
Protein Size (# AA) 161 amino acids
Molecular Weight 17kDa
NCBI Gene Id 3400
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Alias Symbols IDB4, bHLHb27
Peptide Sequence Synthetic peptide located within the following region: MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSAAAAAAAAAARC
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Yu,L., et al., (2005) Nat. Genet. 37 (3), 265-274
Description of Target Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995).[supplied by OMIM].
Protein Interactions TCF3; TCF4; ID3; MYOD1; HES1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ID4 (ARP38058_T100) antibody
Blocking Peptide For anti-ID4 (ARP38058_T100) antibody is Catalog # AAP38058 (Previous Catalog # AAPP20232)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human ID4
Uniprot ID P47928
Protein Name DNA-binding protein inhibitor ID-4
Publications

Melcher, R. et al. LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability. Carcinogenesis 32, 636-42 (2011). 21297112

Protein Accession # NP_001537
Purification Protein A purified
Nucleotide Accession # NM_001546
Tested Species Reactivity Human
Gene Symbol ID4
Predicted Species Reactivity Human, Mouse, Rat, Dog, Pig, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 85%
Image 1
293T, A549
Host: Rabbit
Target: ID4
Positive control (+): 293T (2T)
Negative control (-): A549 (N03)
Antibody concentration: 3ug/ml
Image 2
HepG2
Host: Rabbit
Target Name: ID4
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12%
Image 3
Human HepG2
WB Suggested Anti-ID4 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate
 

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