Product Number |
ARP38058_T100 |
Product Page |
www.avivasysbio.com/id4-antibody-n-terminal-region-arp38058-t100.html |
Name |
ID4 Antibody - N-terminal region (ARP38058_T100) |
Protein Size (# AA) |
161 amino acids |
Molecular Weight |
17kDa |
NCBI Gene Id |
3400 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein |
Alias Symbols |
IDB4, bHLHb27 |
Peptide Sequence |
Synthetic peptide located within the following region: MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSAAAAAAAAAARC |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Yu,L., et al., (2005) Nat. Genet. 37 (3), 265-274 |
Description of Target |
Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995).[supplied by OMIM]. |
Protein Interactions |
TCF3; TCF4; ID3; MYOD1; HES1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-ID4 (ARP38058_T100) antibody |
Blocking Peptide |
For anti-ID4 (ARP38058_T100) antibody is Catalog # AAP38058 (Previous Catalog # AAPP20232) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human ID4 |
Uniprot ID |
P47928 |
Protein Name |
DNA-binding protein inhibitor ID-4 |
Publications |
Melcher, R. et al. LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability. Carcinogenesis 32, 636-42 (2011). 21297112 |
Protein Accession # |
NP_001537 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_001546 |
Tested Species Reactivity |
Human |
Gene Symbol |
ID4 |
Predicted Species Reactivity |
Human, Mouse, Rat, Dog, Pig, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 85% |
Image 1 | 293T, A549
| Host: Rabbit Target: ID4 Positive control (+): 293T (2T) Negative control (-): A549 (N03) Antibody concentration: 3ug/ml |
|
Image 2 | HepG2
| Host: Rabbit Target Name: ID4 Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12% |
|
Image 3 | Human HepG2
| WB Suggested Anti-ID4 Antibody Titration: 1.25ug/ml ELISA Titer: 1:1562500 Positive Control: HepG2 cell lysate |
|