Product Number |
ARP37713_T100 |
Product Page |
www.avivasysbio.com/g3bp-antibody-n-terminal-region-arp37713-t100.html |
Name |
G3BP Antibody - N-terminal region (ARP37713_T100) |
Protein Size (# AA) |
466 amino acids |
Molecular Weight |
51kDa |
NCBI Gene Id |
10146 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
GTPase activating protein (SH3 domain) binding protein 1 |
Description |
|
Alias Symbols |
G3BP, HDH-VIII |
Peptide Sequence |
Synthetic peptide located within the following region: EVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Kociok,N., et al., (1999) J. Cell. Biochem. 74 (2), 194-201 |
Description of Target |
G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. |
Protein Interactions |
UBC; USP10; FXR2; MDM2; RNF2; BMI1; RPS27L; EDC4; RPL35; RPL14; EIF2B3; EIF3C; PTBP1; PIN1; PA2G4; YBX1; HNRNPU; FAU; SH3RF2; TARDBP; FBXO25; RIOK2; SND1; VCAM1; env; ITGA4; IL7R; FN1; EPHA8; DTX1; PABPC1; PAXIP1; ESR1; APP; NOLC1; TPM4; LCK; CSK; KCND3; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-G3BP1 (ARP37713_T100) antibody |
Blocking Peptide |
For anti-G3BP1 (ARP37713_T100) antibody is Catalog # AAP37713 (Previous Catalog # AAPP09045) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human G3BP |
Uniprot ID |
Q13283 |
Protein Name |
Ras GTPase-activating protein-binding protein 1 |
Publications |
Combined structural, biochemical and cellular evidence demonstrates that both FGDF motifs in alphavirus nsP3 are required for efficient replication. Open Biol. 6, (2016). 27383630
Coxsackievirus counters the host innate immune response by blocking type III interferon expression. J. Gen. Virol. 97, 1-12 (2016). 26935471
Foot-and-Mouth Disease Virus Leader Protease Cleaves G3BP1 and G3BP2 and Inhibits Stress Granule Formation. J Virol. 93, (2019). 30404792
Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Mol Cell. 73, 946-958.e7 (2019). 30661979
Stress granule components G3BP1 and G3BP2 play a proviral role early in Chikungunya virus replication. J Virol. 89, 4457-69 (2015). 25653451 |
Protein Accession # |
NP_005745 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_005754 |
Tested Species Reactivity |
Human |
Gene Symbol |
G3BP1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100% |
Image 1 | Human HepG2
| WB Suggested Anti-G3BP Antibody Titration: 2.5ug/ml ELISA Titer: 1:312500 Positive Control: HepG2 cell lysate |
|
Image 2 | HepG2
| Host: Rabbit Target Name: G3BP Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12% |
|
Image 3 | 293T Cell Lysate, Human Liver
| Host: Rabbit Target: G3BP1 Positive control (+): 293T Cell Lysate (2T) Negative control (-): Human Liver (LI) Antibody concentration: 0.5ug/ml |
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