Product Number |
ARP36647_T100 |
Product Page |
www.avivasysbio.com/mcm2-antibody-n-terminal-region-arp36647-t100.html |
Name |
MCM2 Antibody - N-terminal region (ARP36647_T100) |
Protein Size (# AA) |
904 amino acids |
Molecular Weight |
99kDa |
NCBI Gene Id |
4171 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Minichromosome maintenance complex component 2 |
Alias Symbols |
BM28, CCNL1, CDCL1, cdc19, DFNA70, D3S3194, MITOTIN |
Peptide Sequence |
Synthetic peptide located within the following region: PGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGM |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Yu,Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206 |
Description of Target |
The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. |
Protein Interactions |
HUWE1; MCM6; CHFR; SUMO2; MCMBP; UBC; SUMO1; MDM2; MCM7; MCM4; HSP90AB1; ACLY; PRRC1; HYOU1; CAP1; NCL; POLA1; TONSL; SNF8; ELL; TCEA1; POLR2A; CDK6; CDK4; CDC6; MCM10; ORC6; CCNB2; VCAM1; P2RX4; ORC5; ORC2; MCM5; MCM3; ITGA4; FN1; CSNK2A1; CDKN2A; DBF4; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-MCM2 (ARP36647_T100) antibody |
Blocking Peptide |
For anti-MCM2 (ARP36647_T100) antibody is Catalog # AAP36647 (Previous Catalog # AAPP07904) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human MCM2 |
Uniprot ID |
P49736 |
Protein Name |
DNA replication licensing factor MCM2 |
Publications |
Rizwani, W., Alexandrow, M. & Chellappan, S. Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication. Cell Cycle 8, 1621-9 (2009). 19377303 |
Sample Type Confirmation |
MCM2 is supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_004517 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_004526 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
MCM2 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93% |
Image 1 | Human Jurkat
| WB Suggested Anti-MCM2 Antibody Titration: 1.25ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysateMCM2 is supported by BioGPS gene expression data to be expressed in Jurkat |
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Image 2 | mouse neural stem
| WB Suggested Anti-MCM2 Antibody Positive Control: Lane 1: 5ug mouse neural stem cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti rabbit - IR-dye Secondry Antibody Dilution : 1:10,000 Submitted by: Anonymous |
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