MCM2 Antibody - N-terminal region (ARP36647_T100)

Data Sheet
 
Product Number ARP36647_T100
Product Page www.avivasysbio.com/mcm2-antibody-n-terminal-region-arp36647-t100.html
Name MCM2 Antibody - N-terminal region (ARP36647_T100)
Protein Size (# AA) 904 amino acids
Molecular Weight 99kDa
NCBI Gene Id 4171
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Minichromosome maintenance complex component 2
Alias Symbols BM28, CCNL1, CDCL1, cdc19, DFNA70, D3S3194, MITOTIN
Peptide Sequence Synthetic peptide located within the following region: PGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGM
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Yu,Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206
Description of Target The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Protein Interactions HUWE1; MCM6; CHFR; SUMO2; MCMBP; UBC; SUMO1; MDM2; MCM7; MCM4; HSP90AB1; ACLY; PRRC1; HYOU1; CAP1; NCL; POLA1; TONSL; SNF8; ELL; TCEA1; POLR2A; CDK6; CDK4; CDC6; MCM10; ORC6; CCNB2; VCAM1; P2RX4; ORC5; ORC2; MCM5; MCM3; ITGA4; FN1; CSNK2A1; CDKN2A; DBF4;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-MCM2 (ARP36647_T100) antibody
Blocking Peptide For anti-MCM2 (ARP36647_T100) antibody is Catalog # AAP36647 (Previous Catalog # AAPP07904)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MCM2
Uniprot ID P49736
Protein Name DNA replication licensing factor MCM2
Publications

Rizwani, W., Alexandrow, M. & Chellappan, S. Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication. Cell Cycle 8, 1621-9 (2009). 19377303

Sample Type Confirmation

MCM2 is supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_004517
Purification Protein A purified
Nucleotide Accession # NM_004526
Tested Species Reactivity Human, Mouse
Gene Symbol MCM2
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93%
Image 1
Human Jurkat
WB Suggested Anti-MCM2 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysateMCM2 is supported by BioGPS gene expression data to be expressed in Jurkat
Image 2
mouse neural stem
WB Suggested Anti-MCM2 Antibody
Positive Control: Lane 1: 5ug mouse neural stem cell lysate
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti rabbit - IR-dye
Secondry Antibody Dilution : 1:10,000
Submitted by: Anonymous
 

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