Product Number |
ARP36636_P050 |
Product Page |
www.avivasysbio.com/tjp1-antibody-middle-region-arp36636-p050.html |
Name |
TJP1 Antibody - middle region (ARP36636_P050) |
Protein Size (# AA) |
1668 amino acids |
Molecular Weight |
187kDa |
NCBI Gene Id |
7082 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Tight junction protein 1 (zona occludens 1) |
Alias Symbols |
ZO-1 |
Peptide Sequence |
Synthetic peptide located within the following region: QNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Shen,L., (2008) J. Cell Biol. 181 (4), 683-695 |
Description of Target |
TJP1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. TJP1 may be involved in signal transduction at cell-cell junctions.This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. |
Protein Interactions |
VMP1; RAPGEF2; LATS2; TJP2; PAN2; DTNA; UBC; SCO2; VAPA; VAPB; ZPR1; NOTCH2; NDUFV2; NDUFS6; SUGP1; RTN4; STRN; PARD3; PARD6A; PRKCI; SIRT7; KHDRBS1; ELAVL1; SSTR3; Cdk1; TUBA1C; TUBB4A; VIM; SPTAN1; PLEC; KRT10; KRT8; KRT1; GJA1; FLNA; ANXA6; ACTN1; ACTN |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-TJP1 (ARP36636_P050) antibody |
Additional Information |
IHC Information: Paraffin embedded testis tissue, tested with an antibody dilution of 5 ug/ml. |
Blocking Peptide |
For anti-TJP1 (ARP36636_P050) antibody is Catalog # AAP36636 (Previous Catalog # AAPP07874) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human TJP1 |
Uniprot ID |
Q07157 |
Protein Name |
Tight junction protein ZO-1 |
Protein Accession # |
NP_783297 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_175610 |
Tested Species Reactivity |
Human |
Gene Symbol |
TJP1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Dog: 92%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 87%; Pig: 93%; Rabbit: 100%; Rat: 93% |
Image 1 | Human HepG2
| WB Suggested Anti-TJP1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: HepG2 cell lysate |
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Image 2 | Human Testis
| Testis |
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Image 3 |
| Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
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