TJP1 Antibody - middle region (ARP36636_P050)

Data Sheet
 
Product Number ARP36636_P050
Product Page www.avivasysbio.com/tjp1-antibody-middle-region-arp36636-p050.html
Name TJP1 Antibody - middle region (ARP36636_P050)
Protein Size (# AA) 1668 amino acids
Molecular Weight 187kDa
NCBI Gene Id 7082
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Tight junction protein 1 (zona occludens 1)
Alias Symbols ZO-1
Peptide Sequence Synthetic peptide located within the following region: QNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Shen,L., (2008) J. Cell Biol. 181 (4), 683-695
Description of Target TJP1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. TJP1 may be involved in signal transduction at cell-cell junctions.This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.
Protein Interactions VMP1; RAPGEF2; LATS2; TJP2; PAN2; DTNA; UBC; SCO2; VAPA; VAPB; ZPR1; NOTCH2; NDUFV2; NDUFS6; SUGP1; RTN4; STRN; PARD3; PARD6A; PRKCI; SIRT7; KHDRBS1; ELAVL1; SSTR3; Cdk1; TUBA1C; TUBB4A; VIM; SPTAN1; PLEC; KRT10; KRT8; KRT1; GJA1; FLNA; ANXA6; ACTN1; ACTN
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-TJP1 (ARP36636_P050) antibody
Additional Information IHC Information: Paraffin embedded testis tissue, tested with an antibody dilution of 5 ug/ml.
Blocking Peptide For anti-TJP1 (ARP36636_P050) antibody is Catalog # AAP36636 (Previous Catalog # AAPP07874)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human TJP1
Uniprot ID Q07157
Protein Name Tight junction protein ZO-1
Protein Accession # NP_783297
Purification Affinity Purified
Nucleotide Accession # NM_175610
Tested Species Reactivity Human
Gene Symbol TJP1
Predicted Species Reactivity Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Dog: 92%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 87%; Pig: 93%; Rabbit: 100%; Rat: 93%
Image 1
Human HepG2
WB Suggested Anti-TJP1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate
Image 2
Human Testis
Testis
Image 3

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
 

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