Product Number |
ARP36597_P050 |
Product Page |
www.avivasysbio.com/gja1-antibody-middle-region-arp36597-p050.html |
Name |
GJA1 Antibody - middle region (ARP36597_P050) |
Protein Size (# AA) |
382 amino acids |
Molecular Weight |
43kDa |
NCBI Gene Id |
2697 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Gap junction protein, alpha 1, 43kDa |
Alias Symbols |
HSS, CMDR, CX43, EKVP, GJAL, ODDD, AVSD3, EKVP3, HLHS1, PPKCA |
Peptide Sequence |
Synthetic peptide located within the following region: GSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Feller,L., (2008) Am. J. Med. Genet. A 146A (10), 1350-1353 |
Description of Target |
Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Protein Interactions |
UBC; STAMBP; EPS15; UBQLN4; PSMD4; PSMD2; S100A1; GJA1; CNST; NEDD4; TJP1; HGS; TSG101; SGSM3; GJA5; MAPK7; MAPK3; CAV1; CSNK1D; SRC; PRKCG; PRKCE; PRKCB; PRKCA; MAPK1; NOV; CSK; GJA3; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-GJA1 (ARP36597_P050) antibody |
Blocking Peptide |
For anti-GJA1 (ARP36597_P050) antibody is Catalog # AAP36597 (Previous Catalog # AAPP07836) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human GJA1 |
Uniprot ID |
P17302 |
Protein Name |
Gap junction alpha-1 protein |
Protein Accession # |
NP_000156 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_000165 |
Tested Species Reactivity |
Human, Rat |
Gene Symbol |
GJA1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93% |
Image 1 | Human Liver
| WB Suggested Anti-GJA1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Human Liver |
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Image 2 | Human Thyroid Gland
| Rabbit Anti-GJA1 Antibody Catalog Number: ARP36597_P050 Formalin Fixed Paraffin Embedded Tissue: Human Thyroid Gland Tissue Observed Staining: Plasma membrane Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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Image 3 | Rat cardiac
| Lanes: Lane1: 8 ug total cardiac lysate Lane2: 15 ug total cardiac lysate Lane3: 30 ug total cardiac lysate Lane4: 50 ug total cardiac lysate Primary Antibody Dilution: 1 ug/ml Secondary Antibody:
Secondary Antibody Dilution:
Gene Name: GJA1 Submitted by: Anonymous
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Image 4 | Rat
| Species+tissue/cell type: Total rat cardiac lysate 1: 8 ug total cardiac lysate 2: 15 ug total cardiac lysate 3: 30 ug total cardiac lysate4: 50 ug total cardiac lysate Primary antibody dilution: 1 ug/ml |
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