GJA1 Antibody - middle region (ARP36597_P050)

Data Sheet
 
Product Number ARP36597_P050
Product Page www.avivasysbio.com/gja1-antibody-middle-region-arp36597-p050.html
Name GJA1 Antibody - middle region (ARP36597_P050)
Protein Size (# AA) 382 amino acids
Molecular Weight 43kDa
NCBI Gene Id 2697
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Gap junction protein, alpha 1, 43kDa
Alias Symbols HSS, CMDR, CX43, EKVP, GJAL, ODDD, AVSD3, EKVP3, HLHS1, PPKCA
Peptide Sequence Synthetic peptide located within the following region: GSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Feller,L., (2008) Am. J. Med. Genet. A 146A (10), 1350-1353
Description of Target Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions UBC; STAMBP; EPS15; UBQLN4; PSMD4; PSMD2; S100A1; GJA1; CNST; NEDD4; TJP1; HGS; TSG101; SGSM3; GJA5; MAPK7; MAPK3; CAV1; CSNK1D; SRC; PRKCG; PRKCE; PRKCB; PRKCA; MAPK1; NOV; CSK; GJA3;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-GJA1 (ARP36597_P050) antibody
Blocking Peptide For anti-GJA1 (ARP36597_P050) antibody is Catalog # AAP36597 (Previous Catalog # AAPP07836)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human GJA1
Uniprot ID P17302
Protein Name Gap junction alpha-1 protein
Protein Accession # NP_000156
Purification Affinity Purified
Nucleotide Accession # NM_000165
Tested Species Reactivity Human, Rat
Gene Symbol GJA1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93%
Image 1
Human Liver
WB Suggested Anti-GJA1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Liver
Image 2
Human Thyroid Gland
Rabbit Anti-GJA1 Antibody
Catalog Number: ARP36597_P050
Formalin Fixed Paraffin Embedded Tissue: Human Thyroid Gland Tissue
Observed Staining: Plasma membrane
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 3
Rat cardiac
Lanes:
Lane1: 8 ug total cardiac lysate
Lane2: 15 ug total cardiac lysate
Lane3: 30 ug total cardiac lysate
Lane4: 50 ug total cardiac lysate
Primary Antibody Dilution:
1 ug/ml
Secondary Antibody:

Secondary Antibody Dilution:

Gene Name:
GJA1
Submitted by:
Anonymous
Image 4
Rat
Species+tissue/cell type: Total rat cardiac lysate
1: 8 ug total cardiac lysate
2: 15 ug total cardiac lysate
3: 30 ug total cardiac lysate4: 50 ug total cardiac lysate
Primary antibody dilution: 1 ug/ml
 

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