| Product Number |
ARP36568_T100 |
| Product Page |
www.avivasysbio.com/anxa1-antibody-n-terminal-region-arp36568-t100.html |
| Name |
ANXA1 Antibody - N-terminal region (ARP36568_T100) |
| Protein Size (# AA) |
346 amino acids |
| Molecular Weight |
39 kDa |
| NCBI Gene Id |
301 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Annexin A1 |
| Alias Symbols |
ANX1, LPC1 |
| Peptide Sequence |
Synthetic peptide located within the following region: WFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVD |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Ernst,S., et al., (2004) J. Immunol. 172 (12), 7669-7676 |
| Description of Target |
ANXA1 encodes a protein that belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. |
| Protein Interactions |
UBC; MEIS2; SUMO2; SUMO3; WWOX; ZBTB1; HECW2; YWHAQ; ATP4A; UBD; BAG3; HMGA1; VCAM1; ITGA4; FN1; ATF2; ANXA4; RIPK1; IKBKG; RELA; CUL5; CDK2; SIRT7; ANXA5; ANXA1; KLHL23; LRIF1; OTUB1; C1orf123; CSAD; C14orf1; FAF1; COPS6; CCT7; KMT2B; UNC119; SUMO1; UBE2 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
| Relative Expression (Western Blot) |
 |
|
| Datasheets/Manuals |
Printable datasheet for anti-ANXA1 (ARP36568_T100) antibody |
| Blocking Peptide |
For anti-ANXA1 (ARP36568_T100) antibody is Catalog # AAP36568 (Previous Catalog # AAPP07801) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human ANXA1 |
| Uniprot ID |
P04083 |
| Protein Name |
Annexin RuleBase RU003540 |
| Publications |
Ishida, Y. et al. Carnosol, rosemary ingredient, induces apoptosis in adult T-cell leukemia/lymphoma cells via glutathione depletion: proteomic approach using fluorescent two-dimensional differential gel electrophoresis. Hum. Cell 27, 68-77 (2014). 24323765
Nordon, I. M. et al. Comparative proteomics reveals a systemic vulnerability in the vasculature of patients with abdominal aortic aneurysms. J. Vasc. Surg. 54, 1100-1108.e6 (2011). 21741794 |
| Protein Accession # |
NP_000691 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_000700 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
ANXA1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 79%; Dog: 79%; Guinea Pig: 79%; Human: 100%; Mouse: 83%; Rat: 82% |
| Image 1 | Human Intestine
 | Human Intestine |
|
| Image 2 | Mouse fibroblasts
 | WB Suggested Anti-ANXA1 Antibody
Positive Control: Lane 1: 20ug mouse fibroblast lysates
Primary Antibody Dilution : 1:600
Secondary Antibody : Anti rabbit-HRP
Secondry Antibody Dilution : 1:2000
Submitted by: Anonymous |
|
| Image 3 | Human ACHN
 | Host: Rabbit
Target Name: ANXA1
Sample Tissue: Human ACHN
Antibody Dilution: 1.0ug/ml |
|
| Image 4 | A549, RPMI-8226
 | Host: Rabbit
Target: ANXA1
Positive control (+): A549 (N03)
Negative control (-): RPMI-8226 (N12)
Antibody concentration: 0.5ug/ml |
|
| Image 5 | Western Blot
 | 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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