RAD54B Antibody - middle region (ARP36408_P050)

• Product Number: ARP36408_P050
• Name: RAD54B Antibody - middle region (ARP36408_P050)
• Protein Size (# AA): 910 amino acids
• Molecular Weight: 103kDa
• NCBI Gene Id: 25788
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: RAD54 homolog B (S. cerevisiae)
• Alias Symbols: RDH54
• Peptide Sequence: Synthetic peptide located within the following region: NSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Brys,M., (2007) Pol J Pathol 58 (1), 3-6
• Description of Target: RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
• Protein Interactions: TCEANC; ATPAF2; ANKRD36BP1; ENKD1; MYO15B; GMCL1; RAD54B; CDC23; SUMO1; PRKAA1; FUS; HECW2; PSMA1; RAD51; NUDT18; NIF3L1; TCEB3; COPS6; SUMO2; UBC; WRN; ZNF580; LNX1; TRAPPC6A; RIBC2; CCDC33; UBE2I; GORASP2; DYRK2; DMC1; CSNK1E; CD2BP2;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-RAD54B (ARP36408_P050) antibody is Catalog # AAP36408 (Previous Catalog # AAPP08464)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAD54B
• Uniprot ID: Q9Y620
• Protein Name: DNA repair and recombination protein RAD54B
• Sample Type Confirmation:

  RAD54B is supported by BioGPS gene expression data to be expressed in HeLa

• Protein Accession #: NP_036547
• Purification: Affinity Purified
• Nucleotide Accession #: NM_012415
• Tested Species Reactivity: Human
• Gene Symbol: RAD54B
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%
• Image 1: Human Adult Liver
  
  Rabbit Anti-RAD54B Antibody
  Catalog Number: ARP36408_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
  Observed Staining: Nuclear in hepatocytes, strong signal, wide tissue distribution
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  Protocol located in Reviews and Data.
• Image 2: Human HeLa
  
  WB Suggested Anti-RAD54B Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Hela cell lysateRAD54B is supported by BioGPS gene expression data to be expressed in HeLa