DDX21 Antibody - N-terminal region (ARP36374_P050)

Data Sheet
 
Product Number ARP36374_P050
Product Page www.avivasysbio.com/ddx21-antibody-n-terminal-region-arp36374-p050.html
Name DDX21 Antibody - N-terminal region (ARP36374_P050)
Protein Size (# AA) 783 amino acids
Molecular Weight 86kDa
NCBI Gene Id 9188
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name DEAD (Asp-Glu-Ala-Asp) box helicase 21
Alias Symbols GUA, GURDB, RH-II/GU, RH-II/GuA
Peptide Sequence Synthetic peptide located within the following region: MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEET
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Yang,H., et al., (2005) FEBS J. 272 (15), 3788-3802
Description of Target DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
Protein Interactions HUWE1; PA2G4; KPNA3; CEP250; SUMO2; SUMO3; STAU1; IVNS1ABP; UBC; MDM2; LGR4; SUMO1; ERG; WWOX; EED; rev; SRPK2; SRPK3; FBXO6; TARDBP; LYN; AICDA; PAN2; vif; WHSC1; VCAM1; ITGA4; IL7R; FN1; CSNK2A1; YWHAE; SMURF1; H2AFX; APTX; ESR1; FTSJ3; PCDHA2; RRP7A; R
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-DDX21 (ARP36374_P050) antibody
Blocking Peptide For anti-DDX21 (ARP36374_P050) antibody is Catalog # AAP36374 (Previous Catalog # AAPP08432)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human DDX21
Uniprot ID Q9NR30
Protein Name Nucleolar RNA helicase 2
Publications

De Wever, V. et al. Isolation of human mitotic protein phosphatase complexes: identification of a complex between protein phosphatase 1 and the RNA helicase Ddx21. PLoS One 7, e39510 (2012). 22761809

Laux, A. et al. Localization of endogenous morphine-like compounds in the mouse spinal cord. J. Comp. Neurol. 520, 1547-61 (2012). 22174317

Sample Type Confirmation

DDX21 is supported by BioGPS gene expression data to be expressed in HepG2

Protein Accession # NP_004719
Purification Affinity Purified
Nucleotide Accession # NM_004728
Tested Species Reactivity Human
Gene Symbol DDX21
Predicted Species Reactivity Human, Rat, Horse, Yeast
Application WB
Predicted Homology Based on Immunogen Sequence Horse: 79%; Human: 100%; Rat: 91%; Yeast: 83%
Image 1
Human HepG2
WB Suggested Anti-DDX21 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysateDDX21 is supported by BioGPS gene expression data to be expressed in HepG2
 

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