VDAC2 Antibody - N-terminal region (ARP35123_P050)

• Product Number: ARP35123_P050
• Name: VDAC2 Antibody - N-terminal region (ARP35123_P050)
• Protein Size (# AA): 294 amino acids
• Molecular Weight: 32 kDa
• NCBI Gene Id: 7417
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Voltage-dependent anion channel 2
• Alias Symbols: POR
• Peptide Sequence: Synthetic peptide located within the following region: MATHGQTCARPMCIPPSYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Chandra,D., (2005) J. Biol. Chem. 280 (19), 19051-19061
• Description of Target: VDAC2 forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
• Protein Interactions: MDM2; ADRB2; FBXO6; PARK2; PHKG2; PHB; VCAM1; UBC; ITGA4; FN1; ATF2; env; MDC1; ECT2; ACAA2; TRAP1; ATP6V1F; VDAC3; VDAC1; UBE2L3; UBA52; SSBP1; SCP2; RPN1; MPV17; RPSA; HNRNPU; FLOT2; ELAVL1; DDOST; APP; Htt; ZNF454; SFXN3; THOC6; SUGP1; SEPT11; ZFR; ATP
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-VDAC2 (ARP35123_P050) antibody is Catalog # AAP35123 (Previous Catalog # AAPP08406)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human VDAC2
• Uniprot ID: P45880-5
• Protein Name: Voltage-dependent anion-selective channel protein 2
• Publications:

  Hrabakova, R. et al. Cancer cell resistance to aurora kinase inhibitors: identification of novel targets for cancer therapy. J. Proteome Res. 12, 455-69 (2013). 23151231

• Sample Type Confirmation:

  VDAC2 is supported by BioGPS gene expression data to be expressed in HEK293T

• Protein Accession #: NP_003366
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003375
• Tested Species Reactivity: Human
• Gene Symbol: VDAC2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 79%; Zebrafish: 86%
• Image 1: Human Cell Lines
  
  WB Suggested Anti-VDAC2 Antibody
  Titration: 1.6 ug/ml
  Positive Control: human cell lines
• Image 2: Human Bronchial Epithelial Tissue
  
  Rabbit Anti-VDAC2 Antibody
  Catalog Number: ARP35123_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
  Observed Staining: Cytoplasmic
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 3: Human 293T
  
  WB Suggested Anti-VDAC2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Transfected 293TVDAC2 is supported by BioGPS gene expression data to be expressed in HEK293T