Product Number |
ARP35122_T100 |
Product Page |
www.avivasysbio.com/vdac1-antibody-c-terminal-region-arp35122-t100.html |
Name |
VDAC1 Antibody - C-terminal region (ARP35122_T100) |
Protein Size (# AA) |
283 amino acids |
Molecular Weight |
31kDa |
NCBI Gene Id |
7416 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Voltage-dependent anion channel 1 |
Alias Symbols |
PORIN, VDAC-1 |
Peptide Sequence |
Synthetic peptide located within the following region: SAKVNNSSLIGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLGLGLEFQA |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Zheng,Y., et al., (2004) Oncogene 23 (6), 1239-1247 |
Description of Target |
The voltage-dependent anion-selective channel 1 (VDAC1) functions as a channel in membranous structures for the outer mitochondrial membrane, the cell membrane, endosomes, caveolae, the sarcoplasmatic reticulum, synaptosomes, and post-synaptic density fraction. A major function of VDAC1 in the plasma membrane is that of a NADH(-ferricyanide) reductase that may be involved in the maintenance of cellular redox homeostasis. |
Protein Interactions |
UBC; KLHL40; SPRTN; MDM2; ADRB2; ASB14; ASB17; PARK2; BAG3; env; VCAM1; HK1; ATF2; FMNL1; TUBA1B; MDC1; CAV1; Htt; SFXN3; SUGP1; UBAP2; UFM1; ACAD9; MGAT4B; ACAA2; ZMPSTE24; VAPA; ALDH5A1; SF1; VDAC3; VDAC2; UBA52; TIAL1; FLOT2; CDK2; SIRT7; SUMO4; MAPK3; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-VDAC1 (ARP35122_T100) antibody |
Blocking Peptide |
For anti-VDAC1 (ARP35122_T100) antibody is Catalog # AAP35122 (Previous Catalog # AAPP06353) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human VDAC1 |
Uniprot ID |
Q5FVE7 |
Protein Name |
Voltage-dependent anion-selective channel protein 1 |
Publications |
ArduÃno, D. M. et al. Mitochondrial metabolism in Parkinsonâs disease impairs quality control autophagy by hampering microtubule-dependent traffic. Hum. Mol. Genet. 21, 4680-702 (2012). 22843496
Chen, S. et al. Reduced expression of lamin A/C results in modified cell signaling and metabolism coupled with changes in expression of structural proteins. J. Proteome Res. 8, 5196-211 (2009). 19775189
Rousset, F., Nguyen, M. V. C., Grange, L., Morel, F. & Lardy, B. Heme oxygenase-1 regulates matrix metalloproteinase MMP-1 secretion and chondrocyte cell death via Nox4 NADPH oxidase activity in chondrocytes. PLoS One 8, e66478 (2013). 23840483 |
Sample Type Confirmation |
VDAC1 is strongly supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_003365 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_003374 |
Tested Species Reactivity |
Human |
Gene Symbol |
VDAC1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 86% |
Image 1 | Human HepG2
| WB Suggested Anti-VDAC1 Antibody Titration: 1.25ug/ml ELISA Titer: 1:312500 Positive Control: HepG2 cell lysateVDAC1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells |
|
Image 2 | Human Liver
| Rabbit Anti-VDAC1 Antibody Catalog Number: ARP35122 Paraffin Embedded Tissue: Human Liver Cellular Data: Hepatocytes Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
|
Image 3 | Human Stomach Tumor, Human Lung
| Host: Rabbit Target: VDAC1 Positive control (+): Human Stomach Tumor (T-ST) Negative control (-): Human Lung (LU) Antibody concentration: 5ug/ml |
|
Image 4 | Hela, 293T, K562, MDA-MB-231
| Lanes: Lane 1: 50ug HeLa lysate Lane 2: 50ug 293T lysate Lane 3: 50ug K562 lysate Lane 4: 50ug MDA-MB-231 lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:1000 Gene Name: VDAC1 Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences |
|