CLIC1 Antibody - N-terminal region (ARP35045_T100)

• Product Number: ARP35045_T100
• Name: CLIC1 Antibody - N-terminal region (ARP35045_T100)
• Protein Size (# AA): 241 amino acids
• Molecular Weight: 27 kDa
• NCBI Gene Id: 1192
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Chloride intracellular channel 1
• Alias Symbols: G6, CL1C1, NCC27, CLCNL1
• Peptide Sequence: Synthetic peptide located within the following region: GQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIF
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Novarino,G., et al., (2004) J. Neurosci. 24 (23), 5322-5330
• Description of Target: Chloride intracellular channel 1 is a member of the p64 family, a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIon Channel1 encodes a protein that localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.
• Protein Interactions: UBC; PSMD10; SUMO2; SUMO1; NEDD8; FBXO6; ZNF302; NUP62; PRDX4; IKBKG; ZNF184; VCAM1; THRA; RPLP1; NTF4; ITGA4; FN1; ATF2; CPT1A; ATP6V0A1; ATP6V0C; ATP5F1; ALOX5AP; GRK5; MYOC; CDK2; ISG15; ARRB2; SUMO4; LSM1; AKAP9; TRAPPC2;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-CLIC1 (ARP35045_T100) antibody is Catalog # AAP35045 (Previous Catalog # AAPP06272)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CLIC1
• Uniprot ID: Q502X1
• Protein Name: Chloride intracellular channel protein 1
• Publications:

  Yang, J.-Y. et al. Chloride intracellular channel 1 regulates osteoblast differentiation. Bone 45, 1175-85 (2009). 19703605

• Protein Accession #: NP_001279
• Purification: Protein A purified
• Nucleotide Accession #: NM_001288
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: CLIC1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Image 1: Human Jurkat
  
  WB Suggested Anti-CLIC1 Antibody Titration: 1.25ug/ml
  ELISA Titer: 1:312500
  Positive Control: Jurkat cell lysate
• Image 2: Human kidney
  
  Human kidney
• Image 3: Mouse Pancreas
  
  Host: Mouse
  Target Name: CLIC1
  Sample Tissue: Mouse Pancreas
  Antibody Dilution: 1ug/ml
• Image 4: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.