| Product Number |
ARP34527_P050 |
| Product Page |
www.avivasysbio.com/zfr-antibody-middle-region-arp34527-p050.html |
| Name |
ZFR Antibody - middle region (ARP34527_P050) |
| Protein Size (# AA) |
1074 amino acids |
| Molecular Weight |
117kDa |
| NCBI Gene Id |
51663 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Zinc finger RNA binding protein |
| Alias Symbols |
ZFR1, SPG71 |
| Peptide Sequence |
Synthetic peptide located within the following region: RQIHKVLGMDPLPQMSQRFNIHNNRKRRRDSDGVDGFEAEGKKDKKDYDN |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Andersen,J.S., (2005) Nature 433 (7021), 77-83 |
| Description of Target |
ZFR is critical for Staufen 2 isoform specific nucleocytoplasmic shuttling in neurons. |
| Protein Interactions |
UBC; SUMO2; STAU1; ADARB1; RPA3; RPA2; RPA1; RNF2; BMI1; TARDBP; HDAC11; Dlg4; CBX8; WHSC1; VCP; VPS25; USP48; UBAP2; VPS29; DYNC1LI1; THUMPD3; ACAA2; SF1; VDAC2; THY1; S100A10; QARS; PRDX1; HADHA; SERPINH1; ATP6V1E1; COPS5; CUL3; CAND1; HNRNPA1; SIRT7; E |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-ZFR (ARP34527_P050) antibody |
| Blocking Peptide |
For anti-ZFR (ARP34527_P050) antibody is Catalog # AAP34527 (Previous Catalog # AAPP05709) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human ZFR |
| Uniprot ID |
Q96KR1 |
| Protein Name |
Zinc finger RNA-binding protein |
| Sample Type Confirmation |
ZFR is strongly supported by BioGPS gene expression data to be expressed in HT1080 |
| Protein Accession # |
NP_057191 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_016107 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
ZFR |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | heart
 | Rabbit Anti-ZFR Antibody
Catalog Number: ARP34527_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 2.0 sec
Protocol located in Reviews and Data. |
|
| Image 2 | Human Lung
 | Rabbit Anti-ZFR Antibody
Catalog Number: ARP34527
Paraffin Embedded Tissue: Human bronchiole epithelium
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human Pineal Tissue
 | Rabbit Anti-ZFR Antibody
Catalog Number: ARP34527_P050
Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
Observed Staining: Cytoplasmic and nuclear in pinelocytes & intersticial cells
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
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| Image 4 |
 | 25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
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