DPF3 Antibody - middle region (ARP34293_P050)

Data Sheet
 
Product Number ARP34293_P050
Product Page www.avivasysbio.com/dpf3-antibody-middle-region-arp34293-p050.html
Name DPF3 Antibody - middle region (ARP34293_P050)
Protein Size (# AA) 357 amino acids
Molecular Weight 40kDa
NCBI Gene Id 8110
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name D4, zinc and double PHD fingers, family 3
Alias Symbols CERD4, BAF45C
Peptide Sequence Synthetic peptide located within the following region: KDGFTSESTTLEALLRGEGVEKKVDAREEESIQEIQRVLENDENVEEGNE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Hoyal,C.R., (er) J Carcinog 4, 13 (2005)
Description of Target DPF3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). DPF3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.
Protein Interactions SMARCA4; SMARCA2; RELA; NFKB1; SMARCD1; SMARCC1; SMARCB1; ESRRA;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-DPF3 (ARP34293_P050) antibody
Blocking Peptide For anti-DPF3 (ARP34293_P050) antibody is Catalog # AAP34293 (Previous Catalog # AAPP05643)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human DPF3
Uniprot ID Q92784
Protein Name Zinc finger protein DPF3
Publications

Theodorou, M. et al. Identification of a STAT5 target gene, Dpf3, provides novel insights in chronic lymphocytic leukemia. PLoS One 8, e76155 (2013). 24155890

Sample Type Confirmation

DPF3 is supported by BioGPS gene expression data to be expressed in NCIH226

Protein Accession # NP_036206
Purification Affinity Purified
Nucleotide Accession # NM_012074
Tested Species Reactivity Human
Gene Symbol DPF3
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Image 1
Human NCI-H226
Host: Rabbit
Target Name: DPF3
Sample Type: NCI-H226
Antibody Dilution: 1.0ug/mlDPF3 is supported by BioGPS gene expression data to be expressed in NCIH226
Image 2
Human MCF-7
WB Suggested Anti-DPF3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: MCF7 cell lysate
Image 3
Human HT1080
Host: Rabbit
Target Name: DPF3
Sample Type: HT1080
Antibody Dilution: 1.0ug/ml
Image 4
Human MCF7
Host: Rabbit
Target Name: DPF3
Sample Type: MCF7
Antibody Dilution: 1.0ug/ml
Image 5
Human HepG2 Whole Cell
Host: Rabbit
Target Name: DPF3
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 1ug/ml
 

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