XRCC5 Antibody - C-terminal region (ARP34121_T100)

Data Sheet
 
Product Number ARP34121_T100
Product Page www.avivasysbio.com/xrcc5-antibody-c-terminal-region-arp34121-t100.html
Name XRCC5 Antibody - C-terminal region (ARP34121_T100)
Protein Size (# AA) 732 amino acids
Molecular Weight 83kDa
NCBI Gene Id 7520
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Alias Symbols KU80, KUB2, Ku86, NFIV, KARP1, KARP-1
Peptide Sequence Synthetic peptide located within the following region: GITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDM
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Sawchuk,D.J., et al., (2004) J. Biol. Chem. 279 (28), 29821-29831
Description of Target XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Protein Interactions HUWE1; UBC; EHF; ELF3; SUMO2; SUMO3; BRCA1; ATRX; Fbxl16; NEDD8; SUMO1; MDM2; RPA3; RPA2; RPA1; SUZ12; EED; RNF2; BMI1; EIF4B; C1QBP; RPL24; PLCB3; PRMT3; EIF3J; CUL2; CSDE1; SF1; TRMT1; SAMHD1; DNMBP; GANAB; HYOU1; NCL; NARS; MSH2; JUP; HSPA4; PARP1; TAR
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-XRCC5 (ARP34121_T100) antibody
Blocking Peptide For anti-XRCC5 (ARP34121_T100) antibody is Catalog # AAP34121 (Previous Catalog # AAPP05592)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human XRCC5
Uniprot ID Q4VBQ5
Protein Name X-ray repair cross-complementing protein 5
Sample Type Confirmation

XRCC5 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_066964
Purification Protein A purified
Nucleotide Accession # NM_021141
Tested Species Reactivity Human
Gene Symbol XRCC5
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 92%; Dog: 92%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 85%; Rabbit: 93%; Rat: 100%
Image 1
Human Jurkat
WB Suggested Anti-XRCC5 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:12500
Positive Control: Jurkat cell lysateXRCC5 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Image 2
Human
Lanes:
1. 30 ug MiaPaca-2 cell lysate
2. 30 ug Panc-1 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit HRP
Secondary Antibody Dilution:
1:4000
Gene Name:
XRCC5
Submitted by:
Dr. Crissy Dudgeon, Ph.D., CINJ
 

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