Product Number |
ARP34121_T100 |
Product Page |
www.avivasysbio.com/xrcc5-antibody-c-terminal-region-arp34121-t100.html |
Name |
XRCC5 Antibody - C-terminal region (ARP34121_T100) |
Protein Size (# AA) |
732 amino acids |
Molecular Weight |
83kDa |
NCBI Gene Id |
7520 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) |
Alias Symbols |
KU80, KUB2, Ku86, NFIV, KARP1, KARP-1 |
Peptide Sequence |
Synthetic peptide located within the following region: GITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDM |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Sawchuk,D.J., et al., (2004) J. Biol. Chem. 279 (28), 29821-29831 |
Description of Target |
XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
Protein Interactions |
HUWE1; UBC; EHF; ELF3; SUMO2; SUMO3; BRCA1; ATRX; Fbxl16; NEDD8; SUMO1; MDM2; RPA3; RPA2; RPA1; SUZ12; EED; RNF2; BMI1; EIF4B; C1QBP; RPL24; PLCB3; PRMT3; EIF3J; CUL2; CSDE1; SF1; TRMT1; SAMHD1; DNMBP; GANAB; HYOU1; NCL; NARS; MSH2; JUP; HSPA4; PARP1; TAR |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-XRCC5 (ARP34121_T100) antibody |
Blocking Peptide |
For anti-XRCC5 (ARP34121_T100) antibody is Catalog # AAP34121 (Previous Catalog # AAPP05592) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human XRCC5 |
Uniprot ID |
Q4VBQ5 |
Protein Name |
X-ray repair cross-complementing protein 5 |
Sample Type Confirmation |
XRCC5 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_066964 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_021141 |
Tested Species Reactivity |
Human |
Gene Symbol |
XRCC5 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 92%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 85%; Rabbit: 93%; Rat: 100% |
Image 1 | Human Jurkat
| WB Suggested Anti-XRCC5 Antibody Titration: 1.25ug/ml ELISA Titer: 1:12500 Positive Control: Jurkat cell lysateXRCC5 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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Image 2 | Human
| Lanes: 1. 30 ug MiaPaca-2 cell lysate 2. 30 ug Panc-1 cell lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Goat anti-Rabbit HRP Secondary Antibody Dilution: 1:4000 Gene Name: XRCC5 Submitted by: Dr. Crissy Dudgeon, Ph.D., CINJ
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