NPM1 Antibody - N-terminal region (ARP34094_T100)

• Product Number: ARP34094_T100
• Name: NPM1 Antibody - N-terminal region (ARP34094_T100)
• Protein Size (# AA): 294 amino acids
• Molecular Weight: 33kDa
• NCBI Gene Id: 4869
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Alias Symbols: B23, NPM
• Peptide Sequence: Synthetic peptide located within the following region: DMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Falini,B., et al., (2005) N. Engl. J. Med. 352 (3), 254-266
• Description of Target: NPM1 is a member of Nucleophosmin (NPM) family. NPM is a ubiquitously expressed nucleolar phosphoprotein that continuously shuttles between the nucleus and cytoplasm
• Protein Interactions: COX8A; NPM2; HUWE1; UBC; PA2G4; FUS; TP53; AURKA; SUMO2; SUMO3; ELF4; STAU1; CDKN2A; LGR4; SUMO1; MDM2; RPA3; RPA2; RPA1; ERG; rev; TRMT61A; OSBP; KPNA1; LIMCH1; ARMCX3; YWHAQ; PARK2; TARDBP; TUBA1C; TAB2; CEP152; DZIP1; COPG1; STK38; COPE; PARK7; AKAP11;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NPM1 (ARP34094_T100) antibody is Catalog # AAP34094 (Previous Catalog # AAPP05303)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NPM1
• Uniprot ID: P06748
• Protein Name: Nucleophosmin
• Publications:

  Komorek, J. et al. Adenovirus type 5 E1A and E6 proteins of low-risk cutaneous beta-human papillomaviruses suppress cell transformation through interaction with FOXK1/K2 transcription factors. J. Virol. 84, 2719-31 (2010). 20053746

  Lin, S., Coutinho-Mansfield, G., Wang, D., Pandit, S. & Fu, X.-D. The splicing factor SC35 has an active role in transcriptional elongation. Nat. Struct. Mol. Biol. 15, 819-26 (2008). 18641664

• Sample Type Confirmation:

  There is BioGPS gene expression data showing that NPM1 is expressed in HepG2

• Protein Accession #: NP_002511
• Purification: Protein A purified
• Nucleotide Accession #: NM_002520
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: NPM1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 77%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Mouse Spleen
  
  Host: Mouse
  Target Name: NPM1
  Sample Tissue: Mouse Spleen
  Antibody Dilution: 1ug/ml
• Image 2: human cornea
  
  Sample Type: human cornea (frozen)
  Blue: DAPI
  Red: NPM1
  Primary Dilution: 1:100
  Image Submitted By: Geraint Parfitt
  Gavin Herbert Eye Institute See Customer Feedback tab for detailed information.
  
  
• Image 3: Human Lung
  
  Human Lung
• Image 4: Human HepG2
  
  WB Suggested Anti-NPM1 Antibody Titration: 1.0ug/ml
  Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that NPM1 is expressed in HepG2
• Image 5: HepG2
  
  Host: Rabbit
  Target Name: NPM1
  Sample Type: HepG2
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 1.25ug/mL
  Peptide Concentration: 2.0ug/mL
  Lysate Quantity: 25ug/lane
  Gel Concentration: 12%There is BioGPS gene expression data showing that NPM1 is expressed in HepG2