Product Number |
ARP33903_P050 |
Product Page |
www.avivasysbio.com/kif5b-antibody-n-terminal-region-arp33903-p050.html |
Name |
KIF5B Antibody - N-terminal region (ARP33903_P050) |
Protein Size (# AA) |
963 amino acids |
Molecular Weight |
110kDa |
NCBI Gene Id |
3799 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Kinesin family member 5B |
Alias Symbols |
KNS, KINH, KNS1, UKHC, HEL-S-61 |
Peptide Sequence |
Synthetic peptide located within the following region: CNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSST |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Diefenbach,R.J., et al., (2004) Biochem. Biophys. Res. Commun. 319 (3), 987-992 |
Description of Target |
Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. Kinesin consists of heavy and light chains both of which have been documented to bind a variety of potential linker or cargo proteins. KIF5B is an isoform of kinesin heavy chain. |
Protein Interactions |
ZBTB8A; HUWE1; TAX1BP1; VPS52; PRKAR2B; AURKB; STAU1; UBC; TRIM25; EZH2; PSME1; PSMB7; PSMB6; PSMB5; PSMB4; PSMB3; PSMA5; PSMA4; KLC2; SMEK2; RNF20; ELP2; PTPN23; RABGAP1; COPE; AKAP2; COPS5; NUP50; PSMA2; KLC1; EPB41L1; DIAPH1; CAST; ACACA; YWHAE; CFTR; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-KIF5B (ARP33903_P050) antibody |
Blocking Peptide |
For anti-KIF5B (ARP33903_P050) antibody is Catalog # AAP33903 (Previous Catalog # AAPP04974) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human KIF5B |
Uniprot ID |
P33176 |
Protein Name |
Kinesin-1 heavy chain |
Publications |
Turner, L. S. et al. Autophagy is increased in prostate cancer cells overexpressing acid ceramidase and enhances resistance to C6 ceramide. Prostate Cancer Prostatic Dis. 14, 30-7 (2011). 21116286 |
Protein Accession # |
NP_004512 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_004521 |
Tested Species Reactivity |
Human |
Gene Symbol |
KIF5B |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Pig, Zebrafish |
Application |
IHC, WB, IP |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rat: 93%; Zebrafish: 86% |
Image 1 | Human Liver
| Human Liver |
|
Image 2 | Human brain
| WB Suggested Anti-KIF5B Antibody Titration: 0.2-1 ug/ml Positive Control: Human brain |
|
Image 3 | Human MCF7 Whole Cell
| Host: Rabbit Target Name: KIF5B Sample Tissue: Human MCF7 Whole Cell Antibody Dilution: 1ug/ml |
|
Image 4 | Hela, Human liver
| Host: Rabbit Target: KIF5B Positive control (+): Hela (HL) Negative control (-): Human liver (LI) Antibody concentration: 2ug/ml |
|
Image 5 | HEK293 Whole Cell Lysate
| KIF5B was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP33903_P050 with 1:200 dilution. Western blot was performed using ARP33903_P050 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: KIF5B IP with ARP33903_P050 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate. |
|
Image 6 | Human Adrenal
| Rabbit Anti-KIF5B antibody Catalog Number: ARP33903 Formalin Fixed Paraffin Embedded Tissue: Human Adrenal Primary antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20x Exposure Time: 0.5-2.0sec |
|