KIF5B Antibody - N-terminal region (ARP33903_P050)

Data Sheet
 
Product Number ARP33903_P050
Product Page www.avivasysbio.com/kif5b-antibody-n-terminal-region-arp33903-p050.html
Name KIF5B Antibody - N-terminal region (ARP33903_P050)
Protein Size (# AA) 963 amino acids
Molecular Weight 110kDa
NCBI Gene Id 3799
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Kinesin family member 5B
Alias Symbols KNS, KINH, KNS1, UKHC, HEL-S-61
Peptide Sequence Synthetic peptide located within the following region: CNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSST
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Diefenbach,R.J., et al., (2004) Biochem. Biophys. Res. Commun. 319 (3), 987-992
Description of Target Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. Kinesin consists of heavy and light chains both of which have been documented to bind a variety of potential linker or cargo proteins. KIF5B is an isoform of kinesin heavy chain.
Protein Interactions ZBTB8A; HUWE1; TAX1BP1; VPS52; PRKAR2B; AURKB; STAU1; UBC; TRIM25; EZH2; PSME1; PSMB7; PSMB6; PSMB5; PSMB4; PSMB3; PSMA5; PSMA4; KLC2; SMEK2; RNF20; ELP2; PTPN23; RABGAP1; COPE; AKAP2; COPS5; NUP50; PSMA2; KLC1; EPB41L1; DIAPH1; CAST; ACACA; YWHAE; CFTR;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-KIF5B (ARP33903_P050) antibody
Blocking Peptide For anti-KIF5B (ARP33903_P050) antibody is Catalog # AAP33903 (Previous Catalog # AAPP04974)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human KIF5B
Uniprot ID P33176
Protein Name Kinesin-1 heavy chain
Publications

Turner, L. S. et al. Autophagy is increased in prostate cancer cells overexpressing acid ceramidase and enhances resistance to C6 ceramide. Prostate Cancer Prostatic Dis. 14, 30-7 (2011). 21116286

Protein Accession # NP_004512
Purification Affinity Purified
Nucleotide Accession # NM_004521
Tested Species Reactivity Human
Gene Symbol KIF5B
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Pig, Zebrafish
Application IHC, WB, IP
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rat: 93%; Zebrafish: 86%
Image 1
Human Liver
Human Liver
Image 2
Human brain
WB Suggested Anti-KIF5B Antibody Titration: 0.2-1 ug/ml
Positive Control: Human brain
Image 3
Human MCF7 Whole Cell
Host: Rabbit
Target Name: KIF5B
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml
Image 4
Hela, Human liver
Host: Rabbit
Target: KIF5B
Positive control (+): Hela (HL)
Negative control (-): Human liver (LI)
Antibody concentration: 2ug/ml
Image 5
HEK293 Whole Cell Lysate
KIF5B was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP33903_P050 with 1:200 dilution. Western blot was performed using ARP33903_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: KIF5B IP with ARP33903_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.
Image 6
Human Adrenal
Rabbit Anti-KIF5B antibody
Catalog Number: ARP33903
Formalin Fixed Paraffin Embedded Tissue: Human Adrenal
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
 

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