SOX18 Antibody - middle region (ARP33056_P050)

• Product Number: ARP33056_P050
• Name: SOX18 Antibody - middle region (ARP33056_P050)
• Protein Size (# AA): 384 amino acids
• Molecular Weight: 41kDa
• NCBI Gene Id: 54345
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: SRY (sex determining region Y)-box 18
• Alias Symbols: HLTS, HLTRS
• Peptide Sequence: Synthetic peptide located within the following region: RTAPPAAPLAGLYYGTLGTPGPYPGPLSPPPEAPPLESAEPLGPAADLWA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Spataro,V., et al., (2005) Arterioscler. Thromb. Vasc. Biol. 25 (11), 2398-2403
• Description of Target: SOX18 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.
• Protein Interactions: GKAP1; MECP2; MEF2C; HMGB1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  SPR Affinity Characterization
  
• Blocking Peptide: For anti-SOX18 (ARP33056_P050) antibody is Catalog # AAP33056 (Previous Catalog # AAPP04085)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SOX18
• Uniprot ID: P35713
• Protein Name: Transcription factor SOX-18
• Publications:

  Deng, Y., Atri, D., Eichmann, A. & Simons, M. Endothelial ERK signaling controls lymphatic fate specification. J. Clin. Invest. 123, 1202-15 (2013). 23391722

  Duong, T. et al. Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice. Cancer Res. 72, 3105-14 (2012). 22523034

  Park, D.-Y. et al. Lymphatic regulator PROX1 determines Schlemm canal integrity and identity. J. Clin. Invest. 124, 3960-3974 (2014). 25061877

• Protein Accession #: NP_060889
• Purification: Affinity Purified
• Nucleotide Accession #: NM_018419
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: SOX18
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig
• Application: IF, WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%
• Image 1: Mouse lymphatic endothelial cell
  
  lymphatic endothelial cell tissue and primary (1/50 up to 1/500) secondary 1/200
• Image 2: Human Fetal Liver
  
  Host: Rabbit
  Target Name: SOX18
  Sample Tissue: Human Fetal Liver
  Antibody Dilution: 1.0ug/ml
• Image 3: Human kidney
  
  Rabbit Anti-SOX18 Antibody
  Catalog Number: ARP33056
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 4: Mouse Brain
  
  Host: Mouse
  Target Name: SOX18
  Sample Tissue: Mouse Brain
  Antibody Dilution: 1ug/ml
• Image 5: Mouse Testis
  
  Host: Mouse
  Target Name: SOX18
  Sample Tissue: Mouse Testis
  Antibody Dilution: 1ug/ml
• Image 6:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
• Image 7:
  
  25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.