Product Number |
ARP32807_P050 |
Product Page |
www.avivasysbio.com/phb-antibody-c-terminal-region-arp32807-p050.html |
Name |
PHB Antibody - C-terminal region (ARP32807_P050) |
Protein Size (# AA) |
272 amino acids |
Molecular Weight |
30kDa |
NCBI Gene Id |
5245 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Prohibitin |
Alias Symbols |
PHB1, HEL-215, HEL-S-54e |
Peptide Sequence |
Synthetic peptide located within the following region: AEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Fraering,P.C., et al., (2004) Biochemistry 43 (30), 9774-9789 |
Description of Target |
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. |
Protein Interactions |
FUS; HUWE1; SUMO2; SPRTN; STAU1; MDM2; SUZ12; EED; RNF2; PARP1; NAP1L1; COX6C; LONRF3; FBXO6; ADRB2; HDAC5; RAF1; KRAS; RAPH1; IPO11; SMG5; YWHAZ; YWHAB; VDAC2; RPS4X; RPL7; PRKCD; PLEC; NPM1; LMNA; IGHG1; HSP90AB1; GTF3C1; DBN1; COL9A2; CAPZA1; SLC25A5; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-PHB (ARP32807_P050) antibody |
Blocking Peptide |
For anti-PHB (ARP32807_P050) antibody is Catalog # AAP32807 (Previous Catalog # AAPP03826) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human PHB |
Uniprot ID |
P35232 |
Protein Name |
Prohibitin |
Sample Type Confirmation |
PHB is supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_002625 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_002634 |
Tested Species Reactivity |
Human |
Gene Symbol |
PHB |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86% |
Image 1 | Human Stomach
| Human Stomach |
|
Image 2 | Human 293T
| Host: Rabbit Target Name: PHB Sample Tissue: Human 293T Antibody Dilution: 1.0ug/ml |
|
Image 3 | Human Lung Tissue
| PHB antibody - C-terminal region (ARP32807_P050)
Catalog Number: ARP32807_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasm of pneumocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
Image 4 | Human Liver Tissue
| PHB antibody - C-terminal region (ARP32807_P050)
Catalog Number: ARP32807_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
Image 5 | Human Bronchial Epithelial Tissue
| PHB antibody - C-terminal region (ARP32807_P050)
Catalog Number: ARP32807_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasm in Human Bronchial Epithelial Tissue
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
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