PHB Antibody - C-terminal region (ARP32807_P050)

Data Sheet
 
Product Number ARP32807_P050
Product Page www.avivasysbio.com/phb-antibody-c-terminal-region-arp32807-p050.html
Name PHB Antibody - C-terminal region (ARP32807_P050)
Protein Size (# AA) 272 amino acids
Molecular Weight 30kDa
NCBI Gene Id 5245
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Prohibitin
Alias Symbols PHB1, HEL-215, HEL-S-54e
Peptide Sequence Synthetic peptide located within the following region: AEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Fraering,P.C., et al., (2004) Biochemistry 43 (30), 9774-9789
Description of Target Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Protein Interactions FUS; HUWE1; SUMO2; SPRTN; STAU1; MDM2; SUZ12; EED; RNF2; PARP1; NAP1L1; COX6C; LONRF3; FBXO6; ADRB2; HDAC5; RAF1; KRAS; RAPH1; IPO11; SMG5; YWHAZ; YWHAB; VDAC2; RPS4X; RPL7; PRKCD; PLEC; NPM1; LMNA; IGHG1; HSP90AB1; GTF3C1; DBN1; COL9A2; CAPZA1; SLC25A5;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-PHB (ARP32807_P050) antibody
Blocking Peptide For anti-PHB (ARP32807_P050) antibody is Catalog # AAP32807 (Previous Catalog # AAPP03826)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human PHB
Uniprot ID P35232
Protein Name Prohibitin
Sample Type Confirmation

PHB is supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_002625
Purification Affinity Purified
Nucleotide Accession # NM_002634
Tested Species Reactivity Human
Gene Symbol PHB
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
Image 1
Human Stomach
Human Stomach
Image 2
Human 293T
Host: Rabbit
Target Name: PHB
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml
Image 3
Human Lung Tissue
PHB antibody - C-terminal region (ARP32807_P050)
Catalog Number: ARP32807_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasm of pneumocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 4
Human Liver Tissue
PHB antibody - C-terminal region (ARP32807_P050)
Catalog Number: ARP32807_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 5
Human Bronchial Epithelial Tissue
PHB antibody - C-terminal region (ARP32807_P050)
Catalog Number: ARP32807_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasm in Human Bronchial Epithelial Tissue
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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