OLIG2 Antibody - C-terminal region (ARP32753_P050)

• Product Number: ARP32753_P050
• Name: OLIG2 Antibody - C-terminal region (ARP32753_P050)
• Protein Size (# AA): 323 amino acids
• Molecular Weight: 32 kDa
• NCBI Gene Id: 10215
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Oligodendrocyte lineage transcription factor 2
• Alias Symbols: BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19
• Peptide Sequence: Synthetic peptide located within the following region: AAVSSASLPGSGLPSVGSIRPPHGLLKSPSAAAAAPLGGGGGGSGASGGF
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: OLIG2 is a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. OLIG2 is an essential regulator of ventral neuroectodermal progenitor cell fate. It is associated with T-cell acute lymphoblastic leukemia due to a chromosomal translocation t(14;21)(q11.2;q22). OLIG2 might play a role in learning deficits associated with Down syndrome.
• Protein Interactions: CUL3; SRRM1; SOX8; NKX2-2; EP300; SOX10;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-OLIG2 (ARP32753_P050) antibody is Catalog # AAP32753
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human OLIG2
• Uniprot ID: Q13516
• Protein Name: Oligodendrocyte transcription factor 2
• Protein Accession #: NP_005797
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005806
• Tested Species Reactivity: Human
• Gene Symbol: OLIG2
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rat: 93%
• Image 1: Human
  
  Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage Cells
  Dilution: 1:500
• Image 2: Human 293T
  
  Host: Rabbit
  Target Name: OLIG2
  Sample Tissue: Human 293T
  Antibody Dilution: 1.0ug/ml
• Image 3:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.