NOTCH2 Antibody - middle region (ARP32723_P050)

• Product Number: ARP32723_P050
• Name: NOTCH2 Antibody - middle region (ARP32723_P050)
• Protein Size (# AA): 2471 amino acids
• Molecular Weight: 85kDa
• NCBI Gene Id: 4853
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Notch 2
• Alias Symbols: hN2, AGS2, HJCYS
• Peptide Sequence: Synthetic peptide located within the following region: FPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQW
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Troen,G., (er) Haematologica (2008) In press
• Description of Target: NOTCH2 is a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. NOTCH2 is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. NOTCH2 functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development.This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: ASB14; EGFL7; LATS2; ANKRD28; UBC; SMAD1; NOTCH1; SIN3A; SPEN; SEC24C; TJP1; PSMB1; PLAUR; PCMT1; NDUFV2; NDUFV1; MYOC; CRKL; MAML3; MAML2; CNTN1; DLL1; MAML1; GSK3B; DTX1; MFNG; LFNG; JAG2; JAG1; PSEN2; RBPJ; PSEN1; tat;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NOTCH2 (ARP32723_P050) antibody is Catalog # AAP32723 (Previous Catalog # AAPP03737)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NOTCH2
• Uniprot ID: Q04721
• Protein Name: Neurogenic locus notch homolog protein 2
• Publications:

  Tilley, A. E. et al. Down-regulation of the notch pathway in human airway epithelium in association with smoking and chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 179, 457-66 (2009). 19106307

• Sample Type Confirmation:

  There is BioGPS gene expression data showing that NOTCH2 is expressed in 721_B

• Protein Accession #: NP_077719
• Purification: Affinity Purified
• Nucleotide Accession #: NM_024408
• Tested Species Reactivity: Human
• Gene Symbol: NOTCH2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 93%
• Image 1: Human 721_B
  
  WB Suggested Anti-NOTCH2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that NOTCH2 is expressed in 721_B