IKBKB Antibody - N-terminal region (ARP32665_P050)

Data Sheet
 
Product Number ARP32665_P050
Product Page www.avivasysbio.com/ikbkb-antibody-n-terminal-region-arp32665-p050.html
Name IKBKB Antibody - N-terminal region (ARP32665_P050)
Protein Size (# AA) 756 amino acids
Molecular Weight 86kDa
Subunit beta
NCBI Gene Id 3551
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Alias Symbols IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB, IKK-beta
Peptide Sequence Synthetic peptide located within the following region: CITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNN
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Hu,W., (2008) Biochem. J. 412 (1), 35-43
Description of Target NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-203 AL708460.1 9-211 204-3077 AF080158.1 170-3043 3078-3916 AK023193.1 1980-2818
Protein Interactions BCL10; IKBKG; UBC; Snap23; RELA; NFKBIA; MTOR; RPTOR; ROCK1; IKBKB; CDC37; KEAP1; MAP3K7; FLNC; NGFR; EGLN3; SASH1; COPS5; COPS3; PPP1CA; TPX2; TNFRSF1A; AURKA; PRKCQ; MAP3K1; TFAP2C; NLRC5; PYCARD; TAB2; IKBKE; MAP3K14; CHUK; HSPB1; PRKCE; NAA20; TRAF3IP
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-IKBKB (ARP32665_P050) antibody
Blocking Peptide For anti-IKBKB (ARP32665_P050) antibody is Catalog # AAP32665 (Previous Catalog # AAPP03675)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IKBKB
Uniprot ID O14920
Protein Name Inhibitor of nuclear factor kappa-B kinase subunit beta
Sample Type Confirmation

IKBKB is strongly supported by BioGPS gene expression data to be expressed in 721_B

Protein Accession # NP_001547
Purification Affinity Purified
Nucleotide Accession # NM_001556
Tested Species Reactivity Human, Mouse
Gene Symbol IKBKB
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%
Image 1
Human brain
WB Suggested Anti-IKBKB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human brain
Image 2
Human, Mouse
Researcher:Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) GroupApplication:Western blottingSpecies+tissue/cell type:
Lane 1: 100ug mouse liver lysate
Lane 2: 100ug mouse brain lysate
Lane 3: 100ug mouse heart lysate
Lane 4: 100ug mouse kidney lysate
Lane 5: 100ug mouse lung lysate
Lane 6: 100ug mouse thymus lysate
Lane 7: 100ug mouse spleen lysate
Lane 8: 100ug mouse testis lysate
Lane 9: 100ug HeLa cell lysate
Primary antibody dilution:1:1000
Secondary antibody:Anti-rabbit-AP
Secondary antibody dilution:1:10,000
Image 3
Mouse
Sample Type: Primary vascular endothelial cellsProtocol: Loaded 30ug of cell lysate of each. One normal, one cultured with high glucose (which enhances IKK Beta). Expected band is cut. 10% Gel concentration.Primary dilution: 1:1000
Image 4
Human 721_B
Host: Rabbit
Target Name: IKBKB
Sample Type: Human 721_B
Antibody Dilution: 1.0ug/mlIKBKB is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com