Product Number |
ARP32665_P050 |
Product Page |
www.avivasysbio.com/ikbkb-antibody-n-terminal-region-arp32665-p050.html |
Name |
IKBKB Antibody - N-terminal region (ARP32665_P050) |
Protein Size (# AA) |
756 amino acids |
Molecular Weight |
86kDa |
Subunit |
beta |
NCBI Gene Id |
3551 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta |
Alias Symbols |
IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB, IKK-beta |
Peptide Sequence |
Synthetic peptide located within the following region: CITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNN |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Hu,W., (2008) Biochem. J. 412 (1), 35-43 |
Description of Target |
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-203 AL708460.1 9-211 204-3077 AF080158.1 170-3043 3078-3916 AK023193.1 1980-2818 |
Protein Interactions |
BCL10; IKBKG; UBC; Snap23; RELA; NFKBIA; MTOR; RPTOR; ROCK1; IKBKB; CDC37; KEAP1; MAP3K7; FLNC; NGFR; EGLN3; SASH1; COPS5; COPS3; PPP1CA; TPX2; TNFRSF1A; AURKA; PRKCQ; MAP3K1; TFAP2C; NLRC5; PYCARD; TAB2; IKBKE; MAP3K14; CHUK; HSPB1; PRKCE; NAA20; TRAF3IP |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-IKBKB (ARP32665_P050) antibody |
Blocking Peptide |
For anti-IKBKB (ARP32665_P050) antibody is Catalog # AAP32665 (Previous Catalog # AAPP03675) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human IKBKB |
Uniprot ID |
O14920 |
Protein Name |
Inhibitor of nuclear factor kappa-B kinase subunit beta |
Sample Type Confirmation |
IKBKB is strongly supported by BioGPS gene expression data to be expressed in 721_B |
Protein Accession # |
NP_001547 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_001556 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
IKBKB |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100% |
Image 1 | Human brain
| WB Suggested Anti-IKBKB Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Human brain |
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Image 2 | Human, Mouse
| Researcher:Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) GroupApplication:Western blottingSpecies+tissue/cell type: Lane 1: 100ug mouse liver lysate Lane 2: 100ug mouse brain lysate Lane 3: 100ug mouse heart lysate Lane 4: 100ug mouse kidney lysate Lane 5: 100ug mouse lung lysate Lane 6: 100ug mouse thymus lysate Lane 7: 100ug mouse spleen lysate Lane 8: 100ug mouse testis lysate Lane 9: 100ug HeLa cell lysate Primary antibody dilution:1:1000 Secondary antibody:Anti-rabbit-AP Secondary antibody dilution:1:10,000 |
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Image 3 | Mouse
| Sample Type: Primary vascular endothelial cellsProtocol: Loaded 30ug of cell lysate of each. One normal, one cultured with high glucose (which enhances IKK Beta). Expected band is cut. 10% Gel concentration.Primary dilution: 1:1000 |
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Image 4 | Human 721_B
| Host: Rabbit Target Name: IKBKB Sample Type: Human 721_B Antibody Dilution: 1.0ug/mlIKBKB is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells |
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