HDAC2 Antibody - middle region (ARP32619_P050)

• Product Number: ARP32619_P050
• Name: HDAC2 Antibody - middle region (ARP32619_P050)
• Protein Size (# AA): 582 amino acids
• Molecular Weight: 65kDa
• NCBI Gene Id: 3066
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Histone deacetylase 2
• Alias Symbols: HD2, RPD3, YAF1, KDAC2
• Peptide Sequence: Synthetic peptide located within the following region: HKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSN
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.
• Protein Interactions: BRCA1; SUMO1; UBC; SMAD7; SMAD2; MXD1; NCOR2; SUMO2; SFPQ; KDM5A; HDAC1; DDB1; CUL4B; CHFR; KDM1A; KDM5B; NFE2L2; PRDM6; DNMT3B; DNMT1; NACC2; HIST1H4A; HIST1H3A; HIST2H2BE; HIST2H2AC; PRDM1; EED; RNF2; SP1; CHD4; CHD3; ACTL6A; C16orf87; HMG20A; HMG20B; C
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-HDAC2 (ARP32619_P050) antibody is Catalog # AAP32619 (Previous Catalog # AAPP03628)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HDAC2
• Uniprot ID: Q92769
• Protein Name: Histone deacetylase 2
• Sample Type Confirmation:

  HDAC2 is supported by BioGPS gene expression data to be expressed in HEK293T

• Protein Accession #: NP_001518
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001527
• Tested Species Reactivity: Human
• Gene Symbol: HDAC2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: CHIP, IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 100%
• Image 1: HCT116
  
  Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region (ARP32619_P050) and HCT116 Cells
• Image 2: Human 293T
  
  WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: 293T cell lysateHDAC2 is supported by BioGPS gene expression data to be expressed in HEK293T
• Image 3: Human Lung
  
  Rabbit Anti-HDAC2 antibody
  Catalog Number: ARP32619_P050
  Paraffin Embedded Tissue: Human Lung cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 4: Human Adult Placenta
  
  Host: Rabbit
  Target Name: HDAC2
  Sample Type: Human Adult Placenta
  Antibody Dilution: 1.0ug/ml
• Image 5: HCT116
  
  Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.