| Product Number |
ARP32586_P050 |
| Product Page |
www.avivasysbio.com/hipk2-antibody-middle-region-arp32586-p050.html |
| Name |
HIPK2 Antibody - middle region (ARP32586_P050) |
| Protein Size (# AA) |
1198 amino acids |
| Molecular Weight |
120kDa |
| NCBI Gene Id |
28996 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Homeodomain interacting protein kinase 2 |
| Description |
|
| Alias Symbols |
PRO0593 |
| Peptide Sequence |
Synthetic peptide located within the following region: MWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTR |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Description of Target |
HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors. |
| Protein Interactions |
UBC; TP53; SIAH2; XAF1; AATF; SUMO1; MDM2; AXIN1; SIAH1; MBP; DAXX; MKNK1; MECP2; ZBTB4; HIPK2; DCAF7; DYRK1B; MAP3K1; DYRK1A; FBXO3; CBX4; SUMO2; SUMO3; PML; PAX6; CHMP4B; SENP2; SENP1; UBE2I; HIST2H2BE; CXCL1; SIAH1P1; WSB1; MYBL2; MYBL1; NLK; MAP3K7; M |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-HIPK2 (ARP32586_P050) antibody |
| Blocking Peptide |
For anti-HIPK2 (ARP32586_P050) antibody is Catalog # AAP32586 |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of Human HIPK2 |
| Uniprot ID |
Q9H2X6 |
| Protein Name |
Homeodomain-interacting protein kinase 2 |
| Publications |
Choi, D. W. et al. Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WD40 repeat/SOCS box protein WSB-1. J. Biol. Chem. 283, 4682-9 (2008). 18093972
Heat shock modulates the subcellular localization, stability, and activity of HIPK2. Biochem. Biophys. Res. Commun. 472, 580-4 (2016). 26972256
Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011). 21957238
Steinmann, S. et al. v-Myc inhibits C/EBPbeta activity by preventing C/EBPbeta-induced phosphorylation of the co-activator p300. Oncogene 28, 2446-55 (2009). 19448669 |
| Sample Type Confirmation |
HIPK2 is supported by BioGPS gene expression data to be expressed in HepG2 |
| Protein Accession # |
NP_073577 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_022740 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
HIPK2 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
WB, IHC, IP |
| Predicted Homology Based on Immunogen Sequence |
Cow: 86%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 87% |
| Image 1 | Human THP-1 Whole Cell
 | Host: Rabbit
Target Name: HIPK2
Sample Tissue: Human THP-1 Whole Cell
Antibody Dilution: 1ug/ml |
|
| Image 2 | HEK293 Whole Cell Lysate
 | HIPK2 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP32586_P050 with 1:200 dilution. Western blot was performed using ARP32586_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: HIPK2 IP with ARP32586_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate. |
|
| Image 3 | Human Heart
 | Rabbit Anti-HIPK2 Antibody
Catalog Number: ARP32586
Paraffin Embedded Tissue: Human Heart
Cellular Data: Myocardial cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 4 | Human Heart
 | Rabbit Anti-HIPK2 Antibody
Catalog Number: ARP32586
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 5 | Human Brain
 | Rabbit Anti-HIPK2 Antibody
Catalog Number: ARP32586
Paraffin Embedded Tissue: Human neural cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 6 | HepG2
 | Host: Rabbit
Target Name: HIPK2
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1.0ug/mL
Peptide Concentration: 1.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 6%-18%HIPK2 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
| Image 7 | Human HepG2
 | WB Suggested Anti-HIPK2 Antibody Titration: 0.06ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysateHIPK2 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
| Image 8 | Human Pineal Tissue
 | Rabbit Anti-HIPK2 Antibody
Catalog Number: ARP32586_P050
Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
Observed Staining: Nuclear in pinealocytes & intersticial cells
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
|
|
| Image 9 | Human Jurkat
 | WB Suggested Anti-HIPK2 Antibody Titration: 0.1ug/ml
Positive Control: Jurkat Cell Lysate |
|
| Image 10 | Mouse Heart
 | Host: Mouse
Target Name: HIPK2
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml |
|
| Image 11 | heart
 | Rabbit Anti-HIPK2 Antibody
Catalog Number: ARP32586_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Nuclear
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 2.0 sec
Protocol located in Reviews and Data. |
|
