| Product Number |
ARP32512_T100 |
| Product Page |
www.avivasysbio.com/hey1-antibody-c-terminal-region-arp32512-t100.html |
| Name |
HEY1 Antibody - C-terminal region (ARP32512_T100) |
| Protein Size (# AA) |
304 amino acids |
| Molecular Weight |
33 kDa |
| NCBI Gene Id |
23462 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Hairy/enhancer-of-split related with YRPW motif 1 |
| Alias Symbols |
CHF2, OAF1, HERP2, HESR1, HRT-1, NERP2, hHRT1, BHLHb31 |
| Peptide Sequence |
Synthetic peptide located within the following region: PQNGHGNAGTTASPTEPHHQGRLGSAHPEAPALRAPPSGSFGPVLPVVTS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Elagib,K.E., et al., (2004) Mol. Cell. Biol. 24 (17), 7779-7794 |
| Description of Target |
Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. |
| Protein Interactions |
SYVN1; DERL2; HEY2; SEL1L; PRKD2; LAPTM5; SKIL; SMAD9; SMAD3; ARNT; NOTCH1; ELAVL1; FBXW7; HEY1; GATA1; MYOD1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-HEY1 (ARP32512_T100) antibody |
| Blocking Peptide |
For anti-HEY1 (ARP32512_T100) antibody is Catalog # AAP32512 (Previous Catalog # AAPP03515) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human HEY1 |
| Uniprot ID |
Q9Y5J3 |
| Protein Name |
Hairy/enhancer-of-split related with YRPW motif protein 1 |
| Protein Accession # |
NP_036390 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_012258 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
HEY1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 79%; Dog: 86%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 79%; Rat: 93% |
| Image 1 | Human 293T Whole Cell
 | Host: Rabbit
Target Name: HEY1
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 1ug/ml |
|
| Image 2 | Human ACHN Whole Cell
 | Host: Rabbit
Target Name: HEY1
Sample Tissue: Human ACHN Whole Cell
Antibody Dilution: 3ug/ml |
|
| Image 3 | NCI-H226, U937
 | Host: Rabbit
Target: HEY1
Positive control (+): NCI-H226 (N13)
Negative control (-): U937 (N31)
Antibody concentration: 1ug/ml |
|
| Image 4 | Human kidney
 | Rabbit Anti-HEY1 Antibody
Catalog Number: ARP32512
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 5 | Human Lung
 | Rabbit Anti-HEY1 Antibody
Catalog Number: ARP32512
Paraffin Embedded Tissue: Human Lung
Cellular Data: Alveolar cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 6 | Human 293T
 | Host: Rabbit
Target Name: HEY1
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml |
|
| Image 7 |
 | Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
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