REST Antibody - middle region (ARP32478_P050)

• Product Number: ARP32478_P050
• Name: REST Antibody - middle region (ARP32478_P050)
• Protein Size (# AA): 1097 amino acids
• Molecular Weight: 122kDa
• NCBI Gene Id: 5978
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: RE1-silencing transcription factor
• Alias Symbols: WT6, XBR, HGF5, NRSF, DFNA27, GINGF5
• Peptide Sequence: Synthetic peptide located within the following region: PMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Miyajima,F., (er) Genes Brain Behav. (2008) In press
• Description of Target: REST is a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis.This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: BTRC; UBC; FBXW11; CSNK1E; NEDD8; EHMT2; EIF4A3; FBXW4; FBXW7; FBXW5; FBXW8; FBXW2; SIN3A; TERF2; GRIA2; SUB1; GTF2B; Tbp; SIN3B; HDAC1; RCOR1; SP1; SMARCE1; SMARCC2; SMARCA4; CDYL; HDAC5; HDAC4; HTT; NCOR1; KDM5C; RNF2; HDAC2; STMN2; GRM2; CTDSP1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-REST (ARP32478_P050) antibody is Catalog # AAP32478 (Previous Catalog # AAPP03477)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human REST
• Uniprot ID: Q13127
• Protein Name: RE1-silencing transcription factor
• Publications:

  Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins. J. Neurosci. 35, 16443-9 (2015). 26674869

• Sample Type Confirmation:

  REST is supported by BioGPS gene expression data to be expressed in MCF7

• Protein Accession #: NP_005603
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005612
• Tested Species Reactivity: Human
• Gene Symbol: REST
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%
• Image 1: Human A549, HEK293T
  
  REST antibody - middle region (ARP32478_P050) validated by WB using A549 and HEK293T cells at 1: 500.
• Image 2: Human MCF7
  
  Host: Rabbit
  Target Name: REST
  Sample Tissue: Human MCF7
  Antibody Dilution: 1.0ug/ml