PAX3 Antibody - N-terminal region (ARP32445_P050)

• Product Number: ARP32445_P050
• Name: PAX3 Antibody - N-terminal region (ARP32445_P050)
• Protein Size (# AA): 484 amino acids
• Molecular Weight: 53kDa
• NCBI Gene Id: 5077
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Paired box 3
• Alias Symbols: WS1, WS3, CDHS, HUP2
• Peptide Sequence: Synthetic peptide located within the following region: DRNTVPSVSSISRILRSKFGKGEEEEADLERKEAEESEKKAKHSIDGILS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Mercado,G.E., (2008) Genes Chromosomes Cancer 47 (6), 510-520
• Description of Target: PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma.This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.
• Protein Interactions: PCTP; UBC; TAF1; POU3F2; HDAC1; TRIM28; HDAC10; SOX8; WWTR1; PAX3; DAXX; CIB1; SOX10; MEOX2; MEOX1; MITF; MSX1; Rad23b; PSMD4; TBP; IPO13; CNR1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-PAX3 (ARP32445_P050) antibody is Catalog # AAP32445 (Previous Catalog # AAPP03441)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PAX3
• Uniprot ID: Q494Z3
• Protein Name: Paired box protein Pax-3
• Protein Accession #: NP_852123
• Purification: Affinity Purified
• Nucleotide Accession #: NM_181458
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: PAX3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 83%
• Image 1: Human Jurkat Whole Cell
  
  Host: Rabbit
  Target Name: PAX3
  Sample Tissue: Human Jurkat Whole Cell
  Antibody Dilution: 1ug/ml
• Image 2: Mouse Small Intestine
  
  Host: Mouse
  Target Name: PAX3
  Sample Tissue: Mouse Small Intestine
  Antibody Dilution: 1ug/ml
• Image 3: Human HeLa
  
  WB Suggested Anti-PAX3 Antibody Titration: 0.2-1 ug/ml
  Positive Control: Hela cell lysate
• Image 4: Human
  
  Lanes:
  Lane1: 20ug RH30 lysate
  Lane2: 20ug RH30 lysate
  Lane3: 20ug RH41 lysate
  Lane4: 20ug RH41 lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Donkey anti-rabbit HRP
  Secondary Antibody Dilution:
  1:4000
  Gene Name:
  PAX3
  Submitted by:
  Dr Joanna Selfe, Institute of Cancer Research