| Product Number |
ARP32253_P050 |
| Product Page |
www.avivasysbio.com/epas1-antibody-middle-region-arp32253-p050.html |
| Name |
EPAS1 Antibody - middle region (ARP32253_P050) |
| Protein Size (# AA) |
870 amino acids |
| Molecular Weight |
90kDa |
| NCBI Gene Id |
2034 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Endothelial PAS domain protein 1 |
| Description |
|
| Alias Symbols |
HLF, MOP2, ECYT4, HIF2A, PASD2, bHLHe73 |
| Peptide Sequence |
Synthetic peptide located within the following region: ESLFKPHLMAMNSIFDSSGKGAVSEKSNFLFTKLKEEPEELAQLAPTPGD |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Koizume,S., (2008) Biochem. Biophys. Res. Commun. 371 (2), 251-255 |
| Description of Target |
EPAS1 is a transcription factor involved in the induction of oxygen regulated genes. EPAS1 binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. EPAS1 regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. EPAS1 may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. EPAS1 is a potent activator of the Tie-2 tyrosine kinase expression. The activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction of EPAS1 with redox regulatory protein APEX seems to activate CTAD. |
| Protein Interactions |
MAP1LC3A; SQSTM1; LAMP1; BATF2; BATF3; BATF; DDIT3; DBP; NDN; JUN; EPAS1; NPM1; BBS4; BBS2; BBS1; Arnt; VHL; STAT5A; SMAD3; EWSR1; MED11; MED8; EGLN3; PSMD1; PSMB1; MED1; PHB; MITF; HIF1A; EP300; EMD; CTNNB1; CREBBP; EGLN2; DPY30; MED10; MED25; ARID1B; WR |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-EPAS1 (ARP32253_P050) antibody |
| Blocking Peptide |
For anti-EPAS1 (ARP32253_P050) antibody is Catalog # AAP32253 (Previous Catalog # AAPP03234) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human EPAS1 |
| Uniprot ID |
Q99814 |
| Protein Name |
Endothelial PAS domain-containing protein 1 |
| Protein Accession # |
NP_001421 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001430 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
EPAS1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
| Image 1 | Human 293T
 | WB Suggested Anti-EPAS1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate |
|
