GSC Antibody - N-terminal region (ARP32174_P050)

Data Sheet
 
Product Number ARP32174_P050
Product Page www.avivasysbio.com/gsc-antibody-n-terminal-region-arp32174-p050.html
Product Name GSC Antibody - N-terminal region (ARP32174_P050)
Size 100 ul
Gene Symbol GSC
Alias Symbols -
Protein Size (# AA) 257 amino acids
Molecular Weight 28kDa
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
NCBI Gene Id 145258
Host Rabbit
Clonality Polyclonal
Concentration Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Official Gene Full Name Goosecoid homeobox
Peptide Sequence Synthetic peptide located within the following region: FPALHGDSLYGASGGASSDYGAFYPRPVAPGGAGLPAAVSGSRLGYNNYF
Target Reference Kohler,A., (2008) Am. J. Med. Genet. A 146 (1), 117-123
Description of Target GSC regulates chordin (CHRD). GSC may play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis.This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.
Protein Interactions SMAD3; SMAD1; SMAD2; FOXA2;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Tips Information

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Datasheets/Manuals Printable datasheet for anti-GSC (ARP32174_P050) antibody
Protocol
The following related protocols are available on www.avivasysbio.com
Lead Time Domestic: within 1-2 days delivery International: 1-2 days
Blocking Peptide For anti-GSC (ARP32174_P050) antibody is Catalog # AAP32174 (Previous Catalog # AAPP03147)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GSC
Complete computational species homology data Anti-GSC (ARP32174_P050)
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express GSC.
Swissprot Id P56915
Protein Name Homeobox protein goosecoid
Publications

Behl, M; Hsieh, JH; Shafer, TJ; Mundy, WR; Rice, JR; Boyd, WA; Freedman, JH; Hunter, ES; Jarema, KA; Padilla, S; Tice, RR; Use of alternative assays to identify and prioritize organophosphorus flame retardants for potential developmental and neurotoxicity. 52, 181-93 (2015). IHC, WB, Cow, Dog, Human, Mouse, Pig, Rat 26386178

Protein Accession # NP_776248
Purification Affinity Purified
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express GSC.
Nucleotide Accession # NM_173849
Replacement Item This antibody may replace item sc-22234 from Santa Cruz Biotechnology.
Tested Species Reactivity Human
Predicted Species Reactivity Cow, Dog, Human, Mouse, Pig, Rat
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 86%; Dog: 92%; Human: 100%; Mouse: 85%; Pig: 100%; Rat: 85%
Image 1
Human Tonsil
Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody (ARP32174_P050)
Image 2
Human Testis
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody (ARP32174_P050)
Image 3
Human DLD1
Host: Rabbit
Target Name: GSC
Sample Tissue: Human DLD1
Antibody Dilution: 1.0ug/ml
Image 4
Human 786-0 Whole Cell
Host: Rabbit
Target Name: GSC
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 1ug/ml
Image 5
MCF7 Cell Lysate, Human Kidney
Host: Rabbit
Target: GSC
Positive control (+): MCF7 Cell Lysate (N10)
Negative control (-): Human Kidney (KI)
Antibody concentration: 3ug/ml
 

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