FOXA2 Antibody - middle region (ARP31944_P050)

Data Sheet
 
Product Number ARP31944_P050
Product Page www.avivasysbio.com/foxa2-antibody-middle-region-arp31944-p050.html
Name FOXA2 Antibody - middle region (ARP31944_P050)
Protein Size (# AA) 457 amino acids
Molecular Weight 48kDa
NCBI Gene Id 3170
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Forkhead box A2
Alias Symbols HNF3B, TCF3B, HNF-3-beta
Peptide Sequence Synthetic peptide located within the following region: ASQAQLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Yang,G., (2008) Blood 111 (1), 392-401
Description of Target FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young.This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene. This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.
Protein Interactions PPARGC1B; GSC; EN2; OTX2; ONECUT1; NCOA1; TLE1; MIP; HOXA5; LHX1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-FOXA2 (ARP31944_P050) antibody
Blocking Peptide For anti-FOXA2 (ARP31944_P050) antibody is Catalog # AAP31944 (Previous Catalog # AAPP02841)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human FOXA2
Uniprot ID Q9Y261
Protein Name Hepatocyte nuclear factor 3-beta
Publications

Kallas, A., Pook, M., Trei, A. & Maimets, T. SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate. Stem Cells Int. 2014, 298163 (2014). 24707296

Menga, A. et al. Insight into mechanism of in vitro insulin secretion increase induced by antipsychotic clozapine: role of FOXA1 and mitochondrial citrate carrier. Eur. Neuropsychopharmacol. 23, 978-87 (2013). 22959654

Richards, T. J. et al. Allele-specific transactivation of matrix metalloproteinase 7 by FOXA2 and correlation with plasma levels in idiopathic pulmonary fibrosis. Am. J. Physiol. Lung Cell. Mol. Physiol. 302, L746-54 (2012). 22268124

Sample Type Confirmation

FOXA2 is strongly supported by BioGPS gene expression data to be expressed in 721_B, HepG2

Protein Accession # NP_068556
Purification Affinity Purified
Nucleotide Accession # NM_021784
Tested Species Reactivity Human
Gene Symbol FOXA2
Predicted Species Reactivity Human, Mouse, Rat, Dog, Horse, Pig, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Dog: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 77%; Rat: 92%
Image 1
Human HeLa
WB Suggested Anti-FOXA2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Hela cell lysate
Image 2
Human Lung
Sample Type: Human lung tissue protein extractsPrimary Dilution: 1:1000Secondary (Anti-Rabbit HRP sc-2004) Dilution: 1:1000
Image 3
Human 721_B
Host: Rabbit
Target Name: FOXA2
Sample Type: Human 721_B
Antibody Dilution: 1.0ug/mlFOXA2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Image 4
Human HepG2
Host: Rabbit
Target Name: FOXA2
Sample Type: Human HepG2
Antibody Dilution: 1.0ug/mlFOXA2 is strongly supported by BioGPS gene expression data to be expressed in HepG2
Image 5
Human Fetal Brain
Host: Rabbit
Target Name: FOXA2
Sample Type: Human Fetal Brain
Antibody Dilution: 1.0ug/ml
 

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