GLI2 Antibody - middle region : FITC (ARP31885_P050-FITC)

• Product Number: ARP31885_P050-FITC
• Name: GLI2 Antibody - middle region : FITC (ARP31885_P050-FITC)
• Protein Size (# AA): 1258 amino acids
• Molecular Weight: 133kDa
• Conjugation: FITC: Fluorescein Isothiocyanate
• NCBI Gene Id: 2736
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: GLI family zinc finger 2
• Alias Symbols: CJS, HPE9, PHS2, THP1, THP2
• Peptide Sequence: Synthetic peptide located within the following region: RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Reference: Ikram,M.S., et al., (2004) J. Invest. Dermatol. 122 (6), 1503-1509
• Description of Target: GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
• Protein Interactions: SPOP; SKI; HDAC1; STK36; FBXW11; SUFU; ZIC2; ZIC1; CREB1; TBP;
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Blocking Peptide: For anti-GLI2 (ARP31885_P050-FITC) antibody is Catalog # AAP31885 (Previous Catalog # AAPP02680)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2
• Uniprot ID: P10070
• Protein Name: Zinc finger protein GLI2
• Publications:

  Kiprilov, E. N. et al. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. J. Cell Biol. 180, 897-904 (2008). ICC/IF, Human 18332216

  Schneider, S. et al. Expression of the Sonic hedgehog pathway in squamous cell carcinoma of the skin and the mucosa of the head and neck. Head Neck 33, 244-50 (2011). WB, Human 20848446

  Tostar, U., Finta, C., Rahman, M. F.-U., Shimokawa, T. & Zaphiropoulos, P. G. Novel mechanism of action on Hedgehog signaling by a suppressor of fused carboxy terminal variant. PLoS One 7, e37761 (2012). WB, Human 22666390

  Kim, Y. et al. Selective down-regulation of glioma-associated oncogene 2 inhibits the proliferation of hepatocellular carcinoma cells. Cancer Res. 67, 3583-93 (2007). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 17440069

  Kabasawa, Y. et al. Amelogenin positive cells scattered in the interstitial component of odontogenic fibromas. J. Clin. Pathol. 61, 851-5 (2008). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18344235

  Omenetti, A. et al. The hedgehog pathway regulates remodelling responses to biliary obstruction in rats. Gut 57, 1275-82 (2008). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18375471

  Omenetti, A. et al. Hedgehog signaling regulates epithelial-mesenchymal transition during biliary fibrosis in rodents and humans. J. Clin. Invest. 118, 3331-42 (2008). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18802480

  Takanaga, H. et al. Gli2 is a novel regulator of sox2 expression in telencephalic neuroepithelial cells. Stem Cells 27, 165-74 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18927476

  Madison, B. B., McKenna, L. B., Dolson, D., Epstein, D. J. & Kaestner, K. H. FoxF1 and FoxL1 link hedgehog signaling and the control of epithelial proliferation in the developing stomach and intestine. J. Biol. Chem. 284, 5936-44 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19049965

  Wall, D. S. et al. Progenitor cell proliferation in the retina is dependent on Notch-independent Sonic hedgehog/Hes1 activity. J. Cell Biol. 184, 101-12 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19124651

  Syn, W.-K. et al. Role for hedgehog pathway in regulating growth and function of invariant NKT cells. Eur. J. Immunol. 39, 1879-92 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19544307

  Kim, J. E. et al. Sonic hedgehog signaling proteins and ATP-binding cassette G2 are aberrantly expressed in diffuse large B-cell lymphoma. Mod. Pathol. 22, 1312-20 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19593328

  Ho, L. et al. Gli2 and p53 cooperate to regulate IGFBP-3- mediated chondrocyte apoptosis in the progression from benign to malignant cartilage tumors. Cancer Cell 16, 126-36 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19647223

  Kim, W.-Y. et al. GSK-3 is a master regulator of neural progenitor homeostasis. Nat. Neurosci. 12, 1390-7 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19801986

  Nakamura, K. et al. Hedgehog promotes neovascularization in pancreatic cancers by regulating Ang-1 and IGF-1 expression in bone-marrow derived pro-angiogenic cells. PLoS One 5, e8824 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20098680

  Youssef, K. K. et al. Identification of the cell lineage at the origin of basal cell carcinoma. Nat. Cell Biol. 12, 299-305 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20154679

  Peacock, Z. S., Cox, D. & Schmidt, B. L. Involvement of PTCH1 mutations in the calcifying epithelial odontogenic tumor. Oral Oncol. 46, 387-92 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20371205

  Coon, V. et al. Molecular therapy targeting Sonic hedgehog and hepatocyte growth factor signaling in a mouse model of medulloblastoma. Mol. Cancer Ther. 9, 2627-36 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20807782

  Malek, R., Matta, J., Taylor, N., Perry, M. E. & Mendrysa, S. M. The p53 inhibitor MDM2 facilitates Sonic Hedgehog-mediated tumorigenesis and influences cerebellar foliation. PLoS One 6, e17884 (2011). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 21437245

  Jung, I. H., Jung, D. E., Park, Y. N., Song, S. Y. & Park, S. W. Aberrant Hedgehog ligands induce progressive pancreatic fibrosis by paracrine activation of myofibroblasts and ductular cells in transgenic zebrafish. PLoS One 6, e27941 (2011). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 22164219

  Yang, L. et al. Hedgehog signaling activation in the development of squamous cell carcinoma and adenocarcinoma of esophagus. Int. J. Biochem. Mol. Biol. 3, 46-57 (2012). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 22509480

  Bosco-Clément, G. et al. Targeting Gli transcription activation by small molecule suppresses tumor growth. Oncogene 33, 2087-97 (2014). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 23686308

  Li, Y. et al. Gene expression profiling reveals the heterogeneous transcriptional activity of Oct3/4 and its possible interaction with Gli2 in mouse embryonic stem cells. Genomics 102, 456-67 WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 24121003

• Protein Accession #: NP_084655
• Purification: Affinity Purified
• Nucleotide Accession #: NM_030379
• Gene Symbol: GLI2
• Predicted Species Reactivity: Human
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Image 1: