GLI2 Antibody - middle region (ARP31885_P050)

• Product Number: ARP31885_P050
• Name: GLI2 Antibody - middle region (ARP31885_P050)
• Protein Size (# AA): 1258 amino acids
• Molecular Weight: 133kDa
• NCBI Gene Id: 2736
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: GLI family zinc finger 2
• Description:
  View product citations for GLI2 Antibody - middle region (ARP31885_P050) on CiteAb
• Alias Symbols: CJS, HPE9, PHS2, THP1, THP2
• Peptide Sequence: Synthetic peptide located within the following region: RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ikram,M.S., et al., (2004) J. Invest. Dermatol. 122 (6), 1503-1509
• Description of Target: GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
• Protein Interactions: SPOP; SKI; HDAC1; STK36; FBXW11; SUFU; ZIC2; ZIC1; CREB1; TBP;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-GLI2 (ARP31885_P050) antibody is Catalog # AAP31885 (Previous Catalog # AAPP02680)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2
• Uniprot ID: P10070
• Protein Name: Zinc finger protein GLI2
• Publications:

  Histological, cellular and behavioural analyses of effects of chemotherapeutic agent cyclophosphamide in the developing cerebellum. Cell Prolif. 52, e12608 (2019). 30932251

  Impact of Sonic Hedgehog Pathway Expression on Outcome in HPV Negative Head and Neck Carcinoma Patients after Surgery and Adjuvant Radiotherapy. PLoS ONE. 11, e0167665 (2016). 27918595

• Protein Accession #: NP_084655
• Purification: Affinity Purified
• Nucleotide Accession #: NM_030379
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: GLI2
• Predicted Species Reactivity: Human
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Image 1: Human Intestine
  
  Rabbit Anti-GLI2 Antibody
  Catalog Number: ARP31885
  Paraffin Embedded Tissue: Human Intestine
  Cellular Data: Epithelial cells of intestinal gland
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Human Heart
  
  Rabbit Anti-GLI2 Antibody
  Catalog Number: ARP31885
  Paraffin Embedded Tissue: Human Heart
  Cellular Data: Myocardial cells
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3: Mouse Gut
  
  Sample Type:
  Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop
  Primary Antibody Dilution:
  1:50
  Secondary Antibody:
  Goat anti-rabbit-cy3
  Secondary Antibody Dilution:
  1:1500
  Color/Signal Descriptions:
  GLI2: Green Neural crest cells:: Red
  Gene Name:
  GLI2
  Submitted by:
  Anonymous
• Image 4: Human HepG2
  
  Host: Rabbit
  Target Name: GLI2
  Sample Type: HepG2 Whole Cell lysates
  Antibody Dilution: 1.0ug/ml
• Image 5: Jurkat
  
  Host: Rabbit
  Target Name: GLI2
  Sample Type: Jurkat
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 2.5ug/mL
  Peptide Concentration: 4.0ug/mL
  Lysate Quantity: 25ug/lane
  Gel Concentration: 6%-18%
• Image 6: Human RPMI 8226 Whole Cell
  
  Host: Rabbit
  Target Name: GLI2
  Sample Tissue: Human RPMI 8226 Whole Cell
  Antibody Dilution: 1ug/ml