AHR Antibody - N-terminal region (ARP31635_T100)

Data Sheet
 
Product Number ARP31635_T100
Product Page www.avivasysbio.com/ahr-antibody-n-terminal-region-arp31635-t100.html
Name AHR Antibody - N-terminal region (ARP31635_T100)
Protein Size (# AA) 848 amino acids
Molecular Weight 96kDa
NCBI Gene Id 196
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Aryl hydrocarbon receptor
Alias Symbols RP85, bHLHe76
Peptide Sequence Synthetic peptide located within the following region: MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRL
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Marlowe,J.L., et al., (2004) J. Biol. Chem. 279 (28), 29013-29022
Description of Target Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. AHR ligands included a variety of aromatic hydrocarbons.
Protein Interactions GNA13; UBC; NFE2L2; MAF; RB1; EP300; ARNT; NCOA2; NCOA1; TBP; TAF9; TAF6; TAF4; NCOA7; NCOR2; BRCA1; IVNS1ABP; AIP; STUB1; AHR; FOXA1; SUMO2; DDB1; CTNNB1; TAF7; PTGES3; SMARCA4; NEDD8; XPO1; ARNTL; CCNT1; NRIP1; DAP3; AR; CUL4B; GTF2F2; RELA; HSP90AA1; E
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-AHR (ARP31635_T100) antibody
Blocking Peptide For anti-AHR (ARP31635_T100) antibody is Catalog # AAP31635 (Previous Catalog # AAPP02422)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human AHR
Uniprot ID P35869
Protein Name Aryl hydrocarbon receptor
Protein Accession # NP_001612
Purification Protein A purified
Nucleotide Accession # NM_001621
Tested Species Reactivity Human
Gene Symbol AHR
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Image 1
Human HepG2
Lanes:
Lane1: 50 ug HepG2 nuclei extract + benzo[a]pyrene
Lane2: 50 ug HepG2 cytoplasm extract + benzo[a]pyrene
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:1000
Gene Name:
AHR (Arylhydrocarbon receptor)
Submitted by:
Anonymous
Image 2
Human HepG2
AHR antibody - N-terminal region (ARP31635_T100) validated by WB using Human HepG2 Lysate at 2.5ug/ml, 5ug/ml.
Image 3
Human Placenta
WB Suggested Anti-AHR Antibody Titration: 2.5ug/ml
ELISA Titer: 1:12500
Positive Control: Human Placenta
Image 4
Human Urinary Bladder
Rabbit Anti-AHR Antibody
Catalog Number: ARP31635_T100
Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 5
A549, THP-1
Host: Rabbit
Target: AHR
Positive control (+): A549 (N03)
Negative control (-): THP-1 (N30)
Antibody concentration: 5ug/ml
Image 6
Human U937 Whole Cell
Host: Rabbit
Target Name: AHR
Sample Tissue: Human U937 Whole Cell
Antibody Dilution: 1ug/ml
 

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