TFAM Antibody - N-terminal region (ARP31400_P050)

Data Sheet
 
Product Number ARP31400_P050
Product Page www.avivasysbio.com/tfam-antibody-n-terminal-region-arp31400-p050.html
Name TFAM Antibody - N-terminal region (ARP31400_P050)
Protein Size (# AA) 246 amino acids
Molecular Weight 29kDa
NCBI Gene Id 7019
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Transcription factor A, mitochondrial
Description
Alias Symbols TCF6, MTTF1, MTTFA, TCF6L1, TCF6L2, TCF6L3, MTDPS15
Peptide Sequence Synthetic peptide located within the following region: AKTTELIRRIAQRWRELPDSKKKIYQDAYRAEWQVYKEEISRFKEQLTPS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Cruzen,M.E., Gene 362, 125-132 (2005)
Description of Target The function remains unknown.This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Protein Interactions AGTRAP; FBXW11; ARL6IP1; NEDD1; PPP2R1A; GRSF1; UBC; MDM2; SUZ12; EED; PAN2; MYC; FN1; C1QBP; CAND1; COPS5; CUL3; ELAVL1; ICT1; TFB2M; TFB1M; PNP; NRF1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-TFAM (ARP31400_P050) antibody
Blocking Peptide For anti-TFAM (ARP31400_P050) antibody is Catalog # AAP31400 (Previous Catalog # AAPP03075)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TFAM
Uniprot ID Q00059
Protein Name Transcription factor A, mitochondrial
Publications

Alterations of mitochondrial biogenesis in chronic lymphocytic leukemia cells with loss of p53. Mitochondrion. 31, 33-39 (2016). 27650502

Effect of aging on mitochondria and metabolism of bovine granulosa cells. J Reprod Dev. NULL, NULL (2020). 32921645

Kemper, M. F., Stirone, C., Krause, D. N., Duckles, S. P. & Procaccio, V. Genomic and non-genomic regulation of PGC1 isoforms by estrogen to increase cerebral vascular mitochondrial biogenesis and reactive oxygen species protection. Eur. J. Pharmacol. 723, 322-9 (2014). 24275351

Kemper, M. F., Zhao, Y., Duckles, S. P. & Krause, D. N. Endogenous ovarian hormones affect mitochondrial efficiency in cerebral endothelium via distinct regulation of PGC-1 isoforms. J. Cereb. Blood Flow Metab. 33, 122-8 (2013). 23093066

Lu, W. et al. ZNF143 transcription factor mediates cell survival through upregulation of the GPX1 activity in the mitochondrial respiratory dysfunction. Cell Death Dis. 3, e422 (2012). 23152058

Miyazaki, T. et al. Intracellular and extracellular ATP coordinately regulate the inverse correlation between osteoclast survival and bone resorption. J. Biol. Chem. 287, 37808-23 (2012). 22988253

Shi, X. et al. Paradoxical effect of mitochondrial respiratory chain impairment on insulin signaling and glucose transport in adipose cells. J. Biol. Chem. 283, 30658-67 (2008). 18779333

Teodoro, J. S. et al. Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation. Mitochondrion 13, 637-46 (2013). 24041461

Vettor, R. et al. Exercise training boosts eNOS-dependent mitochondrial biogenesis in mouse heart: role in adaptation of glucose metabolism. Am. J. Physiol. Endocrinol. Metab. 306, E519-28 (2014). 24381004

Walters, M. W., Bjork, J. A. & Wallace, K. B. Perfluorooctanoic acid stimulated mitochondrial biogenesis and gene transcription in rats. Toxicology 264, 10-5 (2009). 19616056

Zc3h10 is a novel mitochondrial regulator. EMBO Rep. 19, (2018). 29507079

Zc3h10 regulates adipogenesis by controlling translation and F-actin/mitochondria interaction. J Cell Biol. 220 (2021). 33566069

Protein Accession # NP_003192
Purification Affinity Purified
Nucleotide Accession # NM_003201
Tested Species Reactivity Human, Mouse
Gene Symbol TFAM
Predicted Species Reactivity Human, Mouse, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 92%; Dog: 83%; Guinea Pig: 75%; Horse: 92%; Human: 100%; Mouse: 75%; Pig: 92%; Rabbit: 83%
Image 1
Human Jurkat
WB Suggested Anti-TFAM Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate
Image 2
Mouse bEND.3
TFAM antibody - N-terminal region (ARP31400_P050) validated by WB using bEND3 cell lysate at lane1:10 ug, lane2:20 ug.
Image 3
Human
Lanes:
Lane 1 and 2: HepG2 cell lysate
Lane 3 and 4: Hep3B cell lysate
Lane 5 and 6: K562 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:

Secondary Antibody Dilution:
1:1000
Gene Name:
TFAM
Submitted by:
Anonymous
Image 4
Mouse bEND.3
TFAM antibody - N-terminal region (ARP31400_P050) validated by WB using bEND3 cell lysate at lane1:10 ug, lane2:20 ug.
 

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