| Product Number |
ARP31377_P050 |
| Product Page |
www.avivasysbio.com/fosl1-antibody-middle-region-arp31377-p050.html |
| Name |
FOSL1 Antibody - middle region (ARP31377_P050) |
| Protein Size (# AA) |
271 amino acids |
| Molecular Weight |
29kDa |
| NCBI Gene Id |
8061 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
FOS-like antigen 1 |
| Alias Symbols |
FRA, FRA1, fra-1 |
| Peptide Sequence |
Synthetic peptide located within the following region: TDFLQAETDKLEDEKSGLQREIEELQKQKERLELVLEAHRPICKIPEGAK |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Debinski,W. (2005) Mol. Cancer Res. 3 (4), 237-249 |
| Description of Target |
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. The FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. |
| Protein Interactions |
NME7; CREB5; USF1; LDOC1; JUNB; MAFB; MAF; JUN; DDIT3; ATF2; CEBPG; CEBPE; CEBPA; ATF4; ATF3; PARVG; WFDC1; TAB2; ATXN1; MYC; KPNA1; PSMC3; EP300; CREBBP; TRIM24; UBC; HDAC1; CALCOCO1; ATF1; CREB1; SRF; ELK1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-FOSL1 (ARP31377_P050) antibody |
| Blocking Peptide |
For anti-FOSL1 (ARP31377_P050) antibody is Catalog # AAP31377 (Previous Catalog # AAPP02132) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human FOSL1 |
| Uniprot ID |
P15407 |
| Protein Name |
Fos-related antigen 1 |
| Publications |
Seitz, O., Schürmann, C., Pfeilschifter, J., Frank, S. & Sader, R. Identification of the Fra-1 transcription factor in healing skin flaps transplants: a potential role as a negative regulator of VEGF release from keratinocytes. J. Craniomaxillofac. Surg. 40, 379-86 (2012). 21840727
Yang, L. et al. Biological function and prognostic significance of peroxisome proliferator-activated receptor d in rectal cancer. Clin. Cancer Res. 17, 3760-70 (2011). 21499227 |
| Protein Accession # |
NP_005429 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_005438 |
| Tested Species Reactivity |
Human, Rat |
| Gene Symbol |
FOSL1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 85%; Zebrafish: 85% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-FOSL1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate |
|
| Image 2 | Human Lung
 | Rabbit Anti-FOSL1 Antibody
Catalog Number: ARP31377
Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human Lung
 | Rabbit Anti-FOSL1 Antibody
Catalog Number: ARP31377
Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 4 | Human Urinary Bladder
 | Rabbit Anti-FOSL1 Antibody
Catalog Number: ARP31377_P050
Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue
Observed Staining: Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
| Image 5 | Rat Skeletal Muscle
 | Host: Rat
Target Name: FOSL1
Sample Tissue: Rat Skeletal Muscle
Antibody Dilution: 1ug/ml |
|
| Image 6 | Human Skin
 | Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-FOSL1 antibody (ARP31377_P050) |
|
| Image 7 | Human Skin
 | Human Skin |
|
| Image 8 | Human HepG2 Whole Cell
 | Host: Rabbit
Target Name: FOSL1
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 1ug/ml |
|
