ZEB1 Antibody - N-terminal region (ARP30976_P050)

Data Sheet
 
Product Number ARP30976_P050
Product Page www.avivasysbio.com/zeb1-antibody-n-terminal-region-arp30976-p050.html
Name ZEB1 Antibody - N-terminal region (ARP30976_P050)
Protein Size (# AA) 1124 amino acids
Molecular Weight 124kDa
NCBI Gene Id 6935
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Zinc finger E-box binding homeobox 1
Alias Symbols BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1
Peptide Sequence Synthetic peptide located within the following region: NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
Protein Interactions SUMO1; CDK6; GTF2A1; UBR4; SUMO2; SIRT1; SOX2; CTBP2; CTBP1; SMAD7; SMAD6; SMARCA4; SERPINH1; SMAD3; EP300; DRAP1; KAT5; SMAD2; SMAD1; CDH1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ZEB1 (ARP30976_P050) antibody
Blocking Peptide For anti-ZEB1 (ARP30976_P050) antibody is Catalog # AAPP34123
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1
Uniprot ID P37275
Protein Name Zinc finger E-box-binding homeobox 1
Publications

Kothandaraman, N. et al. E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer. BMC Cancer 10, 64 (2010). 20181230

Li, J. et al. A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells. FEBS Lett. 584, 3198-202 (2010). 20547158

Protein Accession # NP_110378
Purification Affinity Purified
Nucleotide Accession # NM_030751
Tested Species Reactivity Human
Gene Symbol ZEB1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93%; Zebrafish: 100%
Image 1
Human 293T
WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysate
Image 2
Human capan1, HPAF
Lanes:
1. 45ug capan1 cell lysate
2. 45 ug HPAF cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
ZEB1
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE
Image 3
Human Capan1
Sample Type:
Human Capan1 cells (Pancreatic cancer cell line)
Primary Antibody Dilution:
1:300
Secondary Antibody:
Anti-rabbit-AlexaFluor-488
Secondary Antibody Dilution:
1:200
Color/Signal Descriptions:
ZEB: Green DAPI: Blue
Gene Name:
ZEB1
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE
Image 4
Human 786-0 Whole Cell
Host: Rabbit
Target Name: ZEB1
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 1ug/ml
Image 5
Human 786-0 Whole Cell
Host: Rabbit
Target Name: ZEB1
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 1ug/ml
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com