Product Number |
ARP30976_P050 |
Product Page |
www.avivasysbio.com/zeb1-antibody-n-terminal-region-arp30976-p050.html |
Name |
ZEB1 Antibody - N-terminal region (ARP30976_P050) |
Protein Size (# AA) |
1124 amino acids |
Molecular Weight |
124kDa |
NCBI Gene Id |
6935 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Zinc finger E-box binding homeobox 1 |
Alias Symbols |
BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1 |
Peptide Sequence |
Synthetic peptide located within the following region: NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site. |
Protein Interactions |
SUMO1; CDK6; GTF2A1; UBR4; SUMO2; SIRT1; SOX2; CTBP2; CTBP1; SMAD7; SMAD6; SMARCA4; SERPINH1; SMAD3; EP300; DRAP1; KAT5; SMAD2; SMAD1; CDH1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-ZEB1 (ARP30976_P050) antibody |
Blocking Peptide |
For anti-ZEB1 (ARP30976_P050) antibody is Catalog # AAPP34123 |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1 |
Uniprot ID |
P37275 |
Protein Name |
Zinc finger E-box-binding homeobox 1 |
Publications |
Kothandaraman, N. et al. E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer. BMC Cancer 10, 64 (2010). 20181230
Li, J. et al. A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells. FEBS Lett. 584, 3198-202 (2010). 20547158 |
Protein Accession # |
NP_110378 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_030751 |
Tested Species Reactivity |
Human |
Gene Symbol |
ZEB1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93%; Zebrafish: 100% |
Image 1 | Human 293T
| WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate |
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Image 2 | Human capan1, HPAF
| Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-Rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: ZEB1 Submitted by: Dr. Pankaj Singh, UNMC, Omaha, NE
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Image 3 | Human Capan1
| Sample Type: Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution: 1:200Color/Signal Descriptions: ZEB: Green DAPI: BlueGene Name: ZEB1Submitted by: Dr. Pankaj Singh, UNMC, Omaha, NE |
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Image 4 | Human 786-0 Whole Cell
| Host: Rabbit Target Name: ZEB1 Sample Tissue: Human 786-0 Whole Cell Antibody Dilution: 1ug/ml |
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Image 5 | Human 786-0 Whole Cell
| Host: Rabbit Target Name: ZEB1 Sample Tissue: Human 786-0 Whole Cell Antibody Dilution: 1ug/ml |
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