CLK4 Antibody - N-terminal region (ARP30645_P050)

• Product Number: ARP30645_P050
• Name: CLK4 Antibody - N-terminal region (ARP30645_P050)
• Protein Size (# AA): 481 amino acids
• Molecular Weight: 57
• NCBI Gene Id: 57396
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: CDC-like kinase 4
• Alias Symbols: DKFZp686A20267
• Peptide Sequence: Synthetic peptide located within the following region: RSRRSSPKRKRNRHCSSHQSRSKSHRRKRSRSIEDDEEGHLICQSGDVLR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined.
• Protein Interactions: KPNA1; KRTAP10-3; KRTAP10-8; KRTAP10-9; KRTAP10-7; CLK4; SRRM2; AHCYL2; SUB1; RASA4; SRPK1; RNF181; CLASRP; UBL5; HSPB2;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-CLK4 (ARP30645_P050) antibody is Catalog # AAP30645 (Previous Catalog # AAPP01302)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CLK4
• Uniprot ID: Q9HAZ1
• Protein Name: Dual specificity protein kinase CLK4
• Publications:

  Eisenreich, A. et al. Regulation of pro-angiogenic tissue factor expression in hypoxia-induced human lung cancer cells. Oncol. Rep. 30, 462-70 (2013). 23604472

• Protein Accession #: NP_065717
• Purification: Affinity Purified
• Nucleotide Accession #: NM_020666
• Tested Species Reactivity: Human
• Gene Symbol: CLK4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93%; Yeast: 91%
• Image 1: Human HMEC-1
  
  Sample type: human microvascular endothelial cells (25ug)
  Primary Dilution: 1:1000
  Secondary Antibody: Goat anti-Rabbit-HRP
  Secondary Dilution: 1:5000
  Image Submitted by: Andreas Eisenreich 
  Charite Universitatsmedizin Berlin 
• Image 2: Human brain
  
  WB Suggested Anti-CLK4 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: Human brain
• Image 3: Human lung, HEK293
  
  Host: Rabbit
  Target: CLK4
  Positive control (+): Human lung (LU)
  Negative control (-): HEK293 (HEK293)
  Antibody concentration: 1ug/ml
• Image 4: Human PANC1 Whole Cell
  
  Host: Rabbit
  Target Name: CLK4
  Sample Tissue: Human PANC1 Whole Cell
  Antibody Dilution: 1ug/ml