RELA Antibody - middle region (ARP30371_P050)

• Product Number: ARP30371_P050
• Name: RELA Antibody - middle region (ARP30371_P050)
• Protein Size (# AA): 551 amino acids
• Molecular Weight: 60kDa
• NCBI Gene Id: 5970
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: V-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Alias Symbols: p65, CMCU, NFKB3
• Peptide Sequence: Synthetic peptide located within the following region: LVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Wittwer,T. (2008) Biochem. Biophys. Res. Commun. 371 (2), 294-297
• Description of Target: p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1760 M62399.1 8-1767
• Protein Interactions: GAN; CCND1; ABCA1; UBC; TCF4; SRSF7; RELA; REL; PRKDC; PLG; PEX1; NFKB2; NFKB1; MUC3A; KRT5; REV1; TXN2; FBXW11; IL1RN; IKBKB; HSPA5; HDAC1; EP300; CREBBP; MAP3K8; CHEK1; CDKN2A; CDK4; SPATA31A3; POTEJ; POTEE; PIWIL4; DIS3L2; DCD; IKBKG; CHUK; PLA2G4A; ST
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-RELA (ARP30371_P050) antibody is Catalog # AAP30371 (Previous Catalog # AAPS08809)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RELA
• Uniprot ID: Q04206
• Protein Name: Transcription factor p65
• Sample Type Confirmation:

  RELA is supported by BioGPS gene expression data to be expressed in HeLa

• Protein Accession #: NP_068810
• Purification: Affinity Purified
• Nucleotide Accession #: NM_021975
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: RELA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%
• Image 1: Human Fetal Heart
  
  Host: Rabbit
  Target Name: RELA
  Sample Type: Human Fetal Heart
  Antibody Dilution: 1.0ug/ml
• Image 2: Human Fetal Lung
  
  Host: Rabbit
  Target Name: RELA
  Sample Type: Human Fetal Lung
  Antibody Dilution: 1.0ug/ml
• Image 3: Human Adult Placenta
  
  Host: Rabbit
  Target Name: RELA
  Sample Type: Human Adult Placenta
  Antibody Dilution: 1.0ug/ml
• Image 4: Human Adult Placenta
  
  Host: Rabbit
  Target Name: RELA
  Sample Type: Human Adult Placenta
  Antibody Dilution: 1.0ug/ml
• Image 5: Human Hela
  
  Host: Rabbit
  Target Name: RELA
  Sample Type: Human Hela
  Antibody Dilution: 1.0ug/mlRELA is supported by BioGPS gene expression data to be expressed in HeLa
• Image 6: Transfected 293T
  
  WB Suggested Anti-RELA Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Transfected 293T
• Image 7: Mouse Liver
  
  Host: Mouse
  Target Name: RELA
  Sample Tissue: Mouse Liver
  Antibody Dilution: 1ug/ml
• Image 8: Mouse Liver
  
  Host: Rabbit
  Target Name: RELA
  Sample Tissue: Mouse Liver
  Antibody Dilution: 1ug/ml
• Image 9: Human liver tissue
  
  Rabbit Anti-RELA Antibody
  Catalog Number: ARP30371_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
  Observed Staining: Cytoplasm in hepatocytes
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec