| Product Number |
ARP30359_T100 |
| Product Page |
www.avivasysbio.com/dffa-antibody-n-terminal-region-arp30359-t100.html |
| Name |
DFFA Antibody - N-terminal region (ARP30359_T100) |
| Protein Size (# AA) |
331 amino acids |
| Molecular Weight |
37kDa |
| Subunit |
alpha |
| NCBI Gene Id |
1676 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
DNA fragmentation factor, 45kDa, alpha polypeptide |
| Alias Symbols |
DFF1, ICAD, DFF-45 |
| Peptide Sequence |
Synthetic peptide located within the following region: MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDIL |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Yan,B., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1504-1509 |
| Description of Target |
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Protein Interactions |
DFFB; UBC; TUBB; ATG3; ISOC1; BZW2; MAT2B; FERMT2; TUBA1A; CNBP; RPS6KA3; PPP5C; PFAS; MAT2A; EIF6; GAPDH; EIF5; CTPS1; CKB; GZMB; CASP7; CASP3; GZMM; TOM1L2; SEPT5; YWHAZ; YWHAG; PRKAA2; STRN4; NECAB2; CIDEB; EWSR1; NAP1L5; TMBIM4; USP15; HERC1; MCC; BAK |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-DFFA (ARP30359_T100) antibody |
| Blocking Peptide |
For anti-DFFA (ARP30359_T100) antibody is Catalog # AAP30359 (Previous Catalog # AAPS08806) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human DFFA |
| Uniprot ID |
O00273 |
| Protein Name |
DNA fragmentation factor subunit alpha |
| Protein Accession # |
NP_004392 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_004401 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
DFFA |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 83%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 83%; Rat: 92% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-DFFA Antibody Titration: 5.0ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate |
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