AKT2 Antibody - middle region (ARP30321_P050)

• Product Number: ARP30321_P050
• Name: AKT2 Antibody - middle region (ARP30321_P050)
• Protein Size (# AA): 481 amino acids
• Molecular Weight: 56kDa
• NCBI Gene Id: 208
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: V-akt murine thymoma viral oncogene homolog 2
• Alias Symbols: PKBB, PRKBB, HIHGHH, PKBBETA, RAC-BETA
• Peptide Sequence: Synthetic peptide located within the following region: TSEVDTRYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Tan,Y., (2008) Cancer Res. 68 (5), 1296-1302
• Description of Target: AKT2 belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene encoding AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: CLIP3; CHUK; PLEKHO1; PRKCZ; UBC; EGFR; POLR1B; SNX27; PNPLA3; STEAP4; ABCG8; POFUT1; TMED2; NAMPT; SORBS2; SPRR2A; CCL14; PRKDC; MAP3K5; HSP90AA1; APOB; APOA1; APPL1; APP; FSHR; VIM; SKI; GRK5; SH3RF1; ESR1; TTC3; SETDB1; XIAP; AKT1S1; TRIB3; PDPK1; GSK3
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-AKT2 (ARP30321_P050) antibody is Catalog # AAP30321 (Previous Catalog # AAPP00702)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AKT2
• Uniprot ID: P31751
• Protein Name: RAC-beta serine/threonine-protein kinase
• Protein Accession #: NP_001617
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001626
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: AKT2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Zebrafish
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Fetal Muscle
  
  Host: Rabbit
  Target Name: AKT2
  Sample Type: Human Fetal Muscle
  Antibody Dilution: 1.0ug/ml
• Image 2: Human MCF-7
  
  WB Suggested Anti-AKT2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: MCF7 cell lysate
• Image 3: Mouse Pancreas
  
  Host: Mouse
  Target Name: AKT1
  Sample Tissue: Mouse Pancreas
  Antibody Dilution: 1ug/ml
• Image 4: Human thyroid tissue
  
  Rabbit Anti-AKT2 Antibody
  Catalog Number: ARP30321_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Thyroid Tissue
  Observed Staining: Plasma membrane in follicular cells
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 5: Human heart
  
  Rabbit Anti-AKT2 Antibody
  Catalog Number: ARP30321_P050
  Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
  Observed Staining: Cytoplasmic, plasma membrane in intercalated disc
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec