Product Number |
ARP30259_P050 |
Product Page |
www.avivasysbio.com/cdk4-antibody-c-terminal-region-arp30259-p050.html |
Name |
CDK4 Antibody - C-terminal region (ARP30259_P050) |
Protein Size (# AA) |
303 amino acids |
Molecular Weight |
34kDa |
NCBI Gene Id |
1019 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Cyclin-dependent kinase 4 |
Alias Symbols |
CMM3, PSK-J3 |
Peptide Sequence |
Synthetic peptide located within the following region: PRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
Cdk4 is probably involved in the control of the cell cycle. |
Protein Interactions |
RELA; HOOK1; CDKN2D; CDKN2C; CCND3; CCND1; CDKN1A; CCNA2; FKBP5; CDC37; CDKN1B; CDK4; UBC; CDKN2B; CDKN2A; CDK13; TARDBP; CAMK1; PTMA; CDK6; RB1; HSP90AB1; HSP90AA1; HSPA8; KLHL32; TRAP1; UCHL1; SKP1; SHOX2; RBL2; RBL1; PGD; OTX2; MYC; MCM2; IL15RA; GLI1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CDK4 (ARP30259_P050) antibody |
Blocking Peptide |
For anti-CDK4 (ARP30259_P050) antibody is Catalog # AAP30259 |
Uniprot ID |
P11802 |
Protein Name |
Cyclin-dependent kinase 4 |
Sample Type Confirmation |
CDK4 is supported by BioGPS gene expression data to be expressed in HCT116 |
Protein Accession # |
NP_000066 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_000075 |
Tested Species Reactivity |
Human |
Gene Symbol |
CDK4 |
Predicted Species Reactivity |
Human, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Sheep |
Application |
IHC, WB, IP |
Predicted Homology Based on Immunogen Sequence |
Cow: 75%; Dog: 83%; Guinea Pig: 83%; Horse: 83%; Human: 100%; Pig: 92%; Rabbit: 83%; Rat: 83%; Sheep: 75% |
Image 1 | Human HepG2
| WB Suggested Anti-CDK4 Antibody Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell |
|
Image 2 | Human HCT116
| Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: CDK4 Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Friedrich-schiller University Jena CDK4 is supported by BioGPS gene expression data to be expressed in HCT116 |
|
Image 3 | Human HCT116
| Lanes: Lane1: 30 ug untreated human HCT116 cell lysate Lane2-3: 30 ug genotoxic agent treated human HCT116 cell lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: CDK4 Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena CDK4 is supported by BioGPS gene expression data to be expressed in HCT116 |
|
Image 4 | Human Heart
| Rabbit Anti-CDK4 antibody Catalog Number: ARP30259_P050 Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
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Image 5 | HEK293 Whole Cell Lysate
| CDK4 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP30259_P050 with 1:200 dilution. Western blot was performed using ARP30259_P050 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: CDK4 IP with ARP30259_P050 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate. |
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Image 6 | Human A549 Whole Cell
| Host: Rabbit Target Name: CDK4 Sample Tissue: Human A549 Whole Cell Antibody Dilution: 1ug/ml |
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