RAD17 Antibody - C-terminal region (ARP30206_T100)

Data Sheet
 
Product Number ARP30206_T100
Product Page www.avivasysbio.com/rad17-antibody-c-terminal-region-arp30206-t100.html
Name RAD17 Antibody - C-terminal region (ARP30206_T100)
Protein Size (# AA) 584 amino acids
Molecular Weight 66kDa
NCBI Gene Id 5884
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name RAD17 homolog (S. pombe)
Alias Symbols CCYC, R24L, RAD24, HRAD17, RAD17SP
Peptide Sequence Synthetic peptide located within the following region: PTQATVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Tsao,C.C., (2004) EMBO J. 23 (23), 4660-4669
Description of Target RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.
Protein Interactions USP20; UBC; PRMT6; CBX7; CBX6; RAD9A; CDH1; ATR; RFC1; H2AFX; FOXO3; ATM; CLSPN; ALK; RAD9B; POLE; HUS1; RAD1; NHP2L1; MCM7; RFC4; RFC5; RFC3; RFC2; POLE4; POLE3; POLE2; PRKDC;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-RAD17 (ARP30206_T100) antibody
Blocking Peptide For anti-RAD17 (ARP30206_T100) antibody is Catalog # AAP30206 (Previous Catalog # AAPS09009)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human RAD17
Uniprot ID O75943-4
Protein Name Cell cycle checkpoint protein RAD17
Sample Type Confirmation

RAD17 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_579919
Purification Protein A purified
Nucleotide Accession # NM_133341
Tested Species Reactivity Human, Mouse
Gene Symbol RAD17
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Pig
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 83%; Dog: 75%; Human: 100%; Mouse: 75%; Pig: 83%; Rat: 75%
Image 1
Human Jurkat
WB Suggested Anti-RAD17 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysateRAD17 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Image 2
Hela, HEK293T, Xenopus laevis egg extract, mouse embryonic
WB Suggested Anti-RAD17 Antibody
Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate
Primary Antibody Dilution : 1:500
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:3000
Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS
 

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