Product Number |
ARP30206_T100 |
Product Page |
www.avivasysbio.com/rad17-antibody-c-terminal-region-arp30206-t100.html |
Name |
RAD17 Antibody - C-terminal region (ARP30206_T100) |
Protein Size (# AA) |
584 amino acids |
Molecular Weight |
66kDa |
NCBI Gene Id |
5884 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
RAD17 homolog (S. pombe) |
Alias Symbols |
CCYC, R24L, RAD24, HRAD17, RAD17SP |
Peptide Sequence |
Synthetic peptide located within the following region: PTQATVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Tsao,C.C., (2004) EMBO J. 23 (23), 4660-4669 |
Description of Target |
RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. |
Protein Interactions |
USP20; UBC; PRMT6; CBX7; CBX6; RAD9A; CDH1; ATR; RFC1; H2AFX; FOXO3; ATM; CLSPN; ALK; RAD9B; POLE; HUS1; RAD1; NHP2L1; MCM7; RFC4; RFC5; RFC3; RFC2; POLE4; POLE3; POLE2; PRKDC; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-RAD17 (ARP30206_T100) antibody |
Blocking Peptide |
For anti-RAD17 (ARP30206_T100) antibody is Catalog # AAP30206 (Previous Catalog # AAPS09009) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human RAD17 |
Uniprot ID |
O75943-4 |
Protein Name |
Cell cycle checkpoint protein RAD17 |
Sample Type Confirmation |
RAD17 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_579919 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_133341 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
RAD17 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Pig |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 83%; Dog: 75%; Human: 100%; Mouse: 75%; Pig: 83%; Rat: 75% |
Image 1 | Human Jurkat
| WB Suggested Anti-RAD17 Antibody Titration: 1.25ug/ml ELISA Titer: 1:62500 Positive Control: Jurkat cell lysateRAD17 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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Image 2 | Hela, HEK293T, Xenopus laevis egg extract, mouse embryonic
| WB Suggested Anti-RAD17 Antibody Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate Primary Antibody Dilution : 1:500 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000 Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS |
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