HSPB1 Antibody - C-terminal region (ARP30177_T100)

Data Sheet
 
Product Number ARP30177_T100
Product Page www.avivasysbio.com/hspb1-antibody-c-terminal-region-arp30177-t100.html
Name HSPB1 Antibody - C-terminal region (ARP30177_T100)
Protein Size (# AA) 205 amino acids
Molecular Weight 23kDa
NCBI Gene Id 3315
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Heat shock 27kDa protein 1
Alias Symbols CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102
Peptide Sequence Synthetic peptide located within the following region: SLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Boncinelli,E., (2006) J. Biol. Chem. 281 (12), 8169-8174
Description of Target HSPB1, like the other heat shock proteins, is part of a complex system of molecular chaperones in epidermal keratinocytes.
Protein Interactions PCSK9; HUWE1; UBC; GATA1; MAPKAPK2; AKT1; CRYAB; MAP3K7; SUMO2; HAUS1; CEP57; AARSD1; BAG3; IRS4; HSF1; LCE3A; TGFB1I1; SUMO1; NEDD8; MDM2; ASB6; ASB10; ASB17; WWOX; ASB4; ZBTB1; RPA3; RPA2; RPA1; EZH2; ESR1; SOX2; NPM1; PLG; CS; MAPKAPK3; INS; HSPB1; SGA
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-HSPB1 (ARP30177_T100) antibody
Blocking Peptide For anti-HSPB1 (ARP30177_T100) antibody is Catalog # AAP30177 (Previous Catalog # AAPS08907)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human HSPB1
Uniprot ID P04792
Protein Name Heat shock protein beta-1
Publications

Heat Shock Protein 27 is down-regulated in Ballooned Hepatocytes of Patients with Nonalcoholic Steatohepatitis (NASH). Sci Rep. 6, 22528 (2016). 26935030

Narjoz, C. et al. Genomic consequences of cytochrome P450 2C9 overexpression in human hepatoma cells. Chem. Res. Toxicol. 22, 779-87 (2009). 19445531

Protein Accession # NP_001531
Purification Protein A purified
Nucleotide Accession # NM_001540
Tested Species Reactivity Human
Gene Symbol HSPB1
Predicted Species Reactivity Human
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Human: 100%
Image 1
Human HepG2
WB Suggested Anti-HSPB1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
Image 2
Human Heart Muscle
Rabbit Anti-HSPB1 Antibody
Catalog Number: ARP30177_T100
Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 3
Human kidney
Human kidney
 

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