Product Number |
ARP30177_T100 |
Product Page |
www.avivasysbio.com/hspb1-antibody-c-terminal-region-arp30177-t100.html |
Name |
HSPB1 Antibody - C-terminal region (ARP30177_T100) |
Protein Size (# AA) |
205 amino acids |
Molecular Weight |
23kDa |
NCBI Gene Id |
3315 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Heat shock 27kDa protein 1 |
Alias Symbols |
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102 |
Peptide Sequence |
Synthetic peptide located within the following region: SLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Boncinelli,E., (2006) J. Biol. Chem. 281 (12), 8169-8174 |
Description of Target |
HSPB1, like the other heat shock proteins, is part of a complex system of molecular chaperones in epidermal keratinocytes. |
Protein Interactions |
PCSK9; HUWE1; UBC; GATA1; MAPKAPK2; AKT1; CRYAB; MAP3K7; SUMO2; HAUS1; CEP57; AARSD1; BAG3; IRS4; HSF1; LCE3A; TGFB1I1; SUMO1; NEDD8; MDM2; ASB6; ASB10; ASB17; WWOX; ASB4; ZBTB1; RPA3; RPA2; RPA1; EZH2; ESR1; SOX2; NPM1; PLG; CS; MAPKAPK3; INS; HSPB1; SGA |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-HSPB1 (ARP30177_T100) antibody |
Blocking Peptide |
For anti-HSPB1 (ARP30177_T100) antibody is Catalog # AAP30177 (Previous Catalog # AAPS08907) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human HSPB1 |
Uniprot ID |
P04792 |
Protein Name |
Heat shock protein beta-1 |
Publications |
Heat Shock Protein 27 is down-regulated in Ballooned Hepatocytes of Patients with Nonalcoholic Steatohepatitis (NASH). Sci Rep. 6, 22528 (2016). 26935030
Narjoz, C. et al. Genomic consequences of cytochrome P450 2C9 overexpression in human hepatoma cells. Chem. Res. Toxicol. 22, 779-87 (2009). 19445531 |
Protein Accession # |
NP_001531 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_001540 |
Tested Species Reactivity |
Human |
Gene Symbol |
HSPB1 |
Predicted Species Reactivity |
Human |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Human: 100% |
Image 1 | Human HepG2
| WB Suggested Anti-HSPB1 Antibody Titration: 1.25ug/ml ELISA Titer: 1:62500 Positive Control: HepG2 cell lysate |
|
Image 2 | Human Heart Muscle
| Rabbit Anti-HSPB1 Antibody Catalog Number: ARP30177_T100 Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
|
Image 3 | Human kidney
| Human kidney |
|