CALR Antibody - C-terminal region (ARP30114_P050)

Data Sheet
 
Product Number ARP30114_p050
Product Page www.avivasysbio.com/calr-antibody-c-terminal-region-arp30114-p050.html
Name CALR Antibody - C-terminal region (ARP30114_P050)
Protein Size (# AA) 417 amino acids
Molecular Weight 48kDa
NCBI Gene Id 811
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Calreticulin
Alias Symbols RO, CRT, SSA, cC1qR, HEL-S-99n
Peptide Sequence Synthetic peptide located within the following region: FGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDK
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Urade,R., et al., (2004) Biochemistry 43 (27), 8858-8868
Description of Target Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.
Protein Interactions HUWE1; UBC; LGR4; MDM2; RNF2; SUZ12; rev; AARSD1; THG1L; NSUN2; SH3GLB1; EEF2K; ARPC1A; ANP32B; ACTR2; ARPC1B; ARPC3; ARPC5; TPD52L2; SH3GL1; RPA3; RPA1; PRKACA; PFDN5; LPP; PARK2; UPF2; UBD; SOX2; PDIA3; CLN5; ITGA3; HLA-E; HLA-A; CALR; SLC27A2; env; MTN
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-CALR (ARP30114_P050) antibody
Blocking Peptide For anti-CALR (ARP30114_P050) antibody is Catalog # AAP30114 (Previous Catalog # AAPH00290)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CALR
Uniprot ID P27797
Protein Name Calreticulin
Publications

MAPRE1 as a plasma biomarker for early-stage colorectal cancer and adenomas. Cancer Prev Res (Phila). 26342024

Sample Type Confirmation

CALR is strongly supported by BioGPS gene expression data to be expressed in HepG2

Protein Accession # NP_004334
Purification Affinity Purified
Nucleotide Accession # NM_004343
Tested Species Reactivity Human
Gene Symbol CALR
Predicted Species Reactivity Human, Mouse, Rat, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Dog: 100%; Goat: 86%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Yeast: 92%; Zebrafish: 79%
Image 1
Human HepG2
WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysateCALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
Image 2
Human Bronchial Epithelial Tissue
CALR antibody - C-terminal region (ARP30114_P050)
Catalog Number: ARP30114_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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