Product Number |
ARP30114_p050 |
Product Page |
www.avivasysbio.com/calr-antibody-c-terminal-region-arp30114-p050.html |
Name |
CALR Antibody - C-terminal region (ARP30114_P050) |
Protein Size (# AA) |
417 amino acids |
Molecular Weight |
48kDa |
NCBI Gene Id |
811 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Calreticulin |
Alias Symbols |
RO, CRT, SSA, cC1qR, HEL-S-99n |
Peptide Sequence |
Synthetic peptide located within the following region: FGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDK |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Urade,R., et al., (2004) Biochemistry 43 (27), 8858-8868 |
Description of Target |
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. |
Protein Interactions |
HUWE1; UBC; LGR4; MDM2; RNF2; SUZ12; rev; AARSD1; THG1L; NSUN2; SH3GLB1; EEF2K; ARPC1A; ANP32B; ACTR2; ARPC1B; ARPC3; ARPC5; TPD52L2; SH3GL1; RPA3; RPA1; PRKACA; PFDN5; LPP; PARK2; UPF2; UBD; SOX2; PDIA3; CLN5; ITGA3; HLA-E; HLA-A; CALR; SLC27A2; env; MTN |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CALR (ARP30114_P050) antibody |
Blocking Peptide |
For anti-CALR (ARP30114_P050) antibody is Catalog # AAP30114 (Previous Catalog # AAPH00290) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human CALR |
Uniprot ID |
P27797 |
Protein Name |
Calreticulin |
Publications |
MAPRE1 as a plasma biomarker for early-stage colorectal cancer and adenomas. Cancer Prev Res (Phila). 26342024 |
Sample Type Confirmation |
CALR is strongly supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_004334 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_004343 |
Tested Species Reactivity |
Human |
Gene Symbol |
CALR |
Predicted Species Reactivity |
Human, Mouse, Rat, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Goat: 86%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Yeast: 92%; Zebrafish: 79% |
Image 1 | Human HepG2
| WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: HepG2 cell lysateCALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells |
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Image 2 | Human Bronchial Epithelial Tissue
| CALR antibody - C-terminal region (ARP30114_P050)
Catalog Number: ARP30114_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
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