EEA1 Antibody - N-terminal region (ARP30074_P050)

Data Sheet
 
Product Number ARP30074_P050
Product Page www.avivasysbio.com/eea1-antibody-n-terminal-region-arp30074-p050.html
Name EEA1 Antibody - N-terminal region (ARP30074_P050)
Protein Size (# AA) 1411 amino acids
Molecular Weight 162kDa
NCBI Gene Id 8411
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Early endosome antigen 1
Alias Symbols MST105, ZFYVE2, MSTP105
Peptide Sequence Synthetic peptide located within the following region: LTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSAS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Fratti,R.A., et al., (2003) J. Biol. Chem. 278 (47), 46961-46967
Description of Target EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
Protein Interactions UBC; RNF2; BMI1; CFTR; ERBB2; VCP; EEA1; RAB5A; TNK2; RABEP1; RABGEF1; PDCD6IP; PXK; HDAC6; ZFYVE16; USO1; STX6; RAB22A; STX12; TOM1; RAB5C; MAPK14; RAB5B; IGF1R; NSF;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-EEA1 (ARP30074_P050) antibody
Other Applications Image 1 Data IP Suggested Anti-EEA1 antibody
Titration: 2 ug/ml
Positive Control: Rat brain homogenate
Blocking Peptide For anti-EEA1 (ARP30074_P050) antibody is Catalog # AAP30074 (Previous Catalog # AAPH00250)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human EEA1
Uniprot ID Q15075
Protein Name Early endosome antigen 1
Publications

Rab22a controls MHC-I intracellular trafficking and antigen cross-presentation by dendritic cells. EMBO Rep. 17, 1753-1765 (2016). 27861124

Protein Accession # NP_003557
Purification Affinity Purified
Nucleotide Accession # NM_003566
Tested Species Reactivity Human, Rat
Gene Symbol EEA1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application IHC, IP, WB
Predicted Homology Based on Immunogen Sequence Cow: 92%; Dog: 75%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 85%; Rat: 92%
Image 1
Human Placenta
WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta
Image 2
Human Adult Liver
Rabbit Anti-EEA1 Antibody
Catalog Number: ARP30074_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.
Image 3
Rat brain
Amount and Sample Type:
500 ug rat brain homogenate
Amount of IP Antibody:
6 ug
Primary Antibody:
EEA1
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:5000
Gene Name:
EEA1
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
 

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