| Product Number |
ARP30074_P050 |
| Product Page |
www.avivasysbio.com/eea1-antibody-n-terminal-region-arp30074-p050.html |
| Name |
EEA1 Antibody - N-terminal region (ARP30074_P050) |
| Protein Size (# AA) |
1411 amino acids |
| Molecular Weight |
162kDa |
| NCBI Gene Id |
8411 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Early endosome antigen 1 |
| Alias Symbols |
MST105, ZFYVE2, MSTP105 |
| Peptide Sequence |
Synthetic peptide located within the following region: LTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSAS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Fratti,R.A., et al., (2003) J. Biol. Chem. 278 (47), 46961-46967 |
| Description of Target |
EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25. |
| Protein Interactions |
UBC; RNF2; BMI1; CFTR; ERBB2; VCP; EEA1; RAB5A; TNK2; RABEP1; RABGEF1; PDCD6IP; PXK; HDAC6; ZFYVE16; USO1; STX6; RAB22A; STX12; TOM1; RAB5C; MAPK14; RAB5B; IGF1R; NSF; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-EEA1 (ARP30074_P050) antibody |
| Other Applications Image 1 Data |
IP Suggested Anti-EEA1 antibody
Titration: 2 ug/ml
Positive Control: Rat brain homogenate
|
| Blocking Peptide |
For anti-EEA1 (ARP30074_P050) antibody is Catalog # AAP30074 (Previous Catalog # AAPH00250) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human EEA1 |
| Uniprot ID |
Q15075 |
| Protein Name |
Early endosome antigen 1 |
| Publications |
Rab22a controls MHC-I intracellular trafficking and antigen cross-presentation by dendritic cells. EMBO Rep. 17, 1753-1765 (2016). 27861124 |
| Protein Accession # |
NP_003557 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_003566 |
| Tested Species Reactivity |
Human, Rat |
| Gene Symbol |
EEA1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
IHC, IP, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 75%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 85%; Rat: 92% |
| Image 1 | Human Placenta
 | WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta |
|
| Image 2 | Human Adult Liver
 | Rabbit Anti-EEA1 Antibody
Catalog Number: ARP30074_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 2.0 sec
Protocol located in Reviews and Data. |
|
| Image 3 | Rat brain
 | Amount and Sample Type:
500 ug rat brain homogenate
Amount of IP Antibody:
6 ug
Primary Antibody:
EEA1
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:5000
Gene Name:
EEA1
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science |
|
