Sku |
AAP32012 |
Old sku |
AAPP23942 |
Price |
$99.00 |
Name |
MBD1 Peptide (AAP32012) |
Purchase info |
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|
Size |
100 ug |
Gene |
MBD1 |
Alias symbols |
CXXC3, PCM1, RFT |
Gene id |
4152 |
Description of target |
MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21. |
Swissprot id |
Q9UIS9-3 |
Protein accession num |
NP_056669 |
Nucleotide accession num |
NM_015844 |
Protein size |
549 amino acids |
Molecular weight |
60kDa |
Application |
WB |
Partner proteins |
ATF7IP,ATF7IP2,CHAF1A,SETDB1,ATF7IP,ATF7IP2,CBX5,CHAF1A,HDAC3,HIPK3,OASL,PIAS1,PIAS2,SETDB1,SMAD1,SMAD3,SMAD5,SMAD7,SMAD9,SUV39H1,ATF7IP,CBX4,CBX5,CHAF1A,RNF2,Rnf2,SUV39H1 |
Quality control |
The peptide is characterized by mass spectroscopy |
Key reference |
Villa,R., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1400-1405 |
Description |
This is a synthetic peptide designed for use in combination with anti-MBD1 antibody (Catalog #: ARP32012_P050) made by Aviva Systems Biology. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications. Please inquire for more details. |
Product format |
Lyophilized powder |
Reconstitution and storage |
Add 100ul of sterile PBS. Final peptide concentration is 1 mg/ml in PBS. For longer periods of storage, store at -20C. Avoid repeat freeze-thaw cycles. |
Lead time |
Domestic: within 24 hours delivery International: 3-5 business days |
Protocol |
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Tips |
See our General FAQ page. |
Availability |
In Stock |