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Catalog No: OKCD01755 (Formerly GWB-KBBXR8)
Size:96WELLS
Price: $675.00
SKU
OKCD01755
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Datasheets/ManualsPrintable datasheet for SCNN1B ELISA Kit (Human) (OKCD01755)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SCNN1B Microplate96 Wells (12 x 8 Well Strips)
SCNN1B Lyophilized Standard2 x 20 ng
100X Biotinylated SCNN1B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.107 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b). No significant cross-reactivity or interference between Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSCNN1B
Gene Full Namesodium channel epithelial 1 subunit beta
Alias Symbolsamiloride-sensitive sodium channel subunit beta;amiloride-sensitive sodium channel subunit beta 1;BESC1;beta-ENaC;beta-NaCH;ENaCb;ENaCbeta;epithelial Na(+) channel subunit beta;epithelial sodium channel beta-2 subunit;epithelial sodium channel beta-3 subunit;LIDLS1;mutant sodium channel epithelial 1 beta subunit;nasal epithelial sodium channel beta subunit;nonvoltage-gated sodium channel 1 subunit beta;SCNEB;sodium channel epithelial 1 beta subunit;sodium channel, non-voltage-gated 1, beta subunit.
NCBI Gene Id6338
Protein NameAmiloride-sensitive sodium channel subunit beta
Description of TargetSodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Uniprot IDP51168
Protein Accession #NP_000327.2
Nucleotide Accession #NM_000336.2
Protein Size (# AA)640
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