SCIN Antibody (OAGA00980)

Catalog No: OAGA00980

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
OAGA00980 diluted at 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using SCIN(OAGA00980) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: SCIN is a Ca(2+)-dependent actin-severing and -capping protein (Zunino et al., 2001 [PubMed 11568009]).[supplied by OMIM]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Cytoplasm, cytoskeleton
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 281 and 585 of SCIN (Uniprot ID#Q9Y6U3)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: SCIN
• Gene Full Name: Adseverin
• Alias Symbols: KIAA1905 antibody, SCIN antibody, adseverin antibody, scinderin antibody
• NCBI Gene Id: 85477
• Protein Name: Adseverin
• Uniprot ID: Q9Y6U3
• Protein Accession #: NP_001106177.1
• Molecular Weight: 81 kDa