Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKCD00931 (Formerly GWB-KBBNK7)
Size:96WELLS
Price: $675.00
SKU
OKCD00931
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for SCGB1D2 ELISA Kit (Human) (OKCD00931)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)90-102%78-102%84-92%94-105%
EDTA plasma(n=5)78-103%85-96%89-104%82-89%
heparin plasma(n=5)85-103%94-102%96-104%81-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipophilin B, Prostatein Like (LIPB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipophilin B, Prostatein Like (LIPB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipophilin B, Prostatein Like (LIPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipophilin B, Prostatein Like (LIPB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-10298
EDTA plasma(n=5)80-8982
heparin plasma(n=5)78-9588
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipophilin B, Prostatein Like (LIPB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipophilin B, Prostatein Like (LIPB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SCGB1D2 Microplate96 Wells (12 x 8 Well Strips)
SCGB1D2 Lyophilized Standard2 x 20 ng
100X Biotinylated SCGB1D2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.112 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipophilin B, Prostatein Like (LIPB). No significant cross-reactivity or interference between Lipophilin B, Prostatein Like (LIPB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSCGB1D2
Gene Full Namesecretoglobin family 1D member 2
Alias SymbolsLIPB;lipophilin-B;LPHB;LPNB;prostatein-like lipophilin B;secretoglobin family 1D member 2.
NCBI Gene Id10647
Protein NameSecretoglobin family 1D member 2
Description of TargetMay bind androgens and other steroids, may also bind estramustine, a chemotherapeutic agent used for prostate cancer. May be under transcriptional regulation of steroid hormones.
Uniprot IDO95969
Protein Accession #NP_006542
Nucleotide Accession #NM_006551.3
Protein Size (# AA)90
Write Your Own Review
You're reviewing:SCGB1D2 ELISA Kit (Human) (OKCD00931)
Your Rating